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CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

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Product Details

Summary
Reactivity Mu, Hu(-)Species Glossary
Applications Flow, ICC/IF
Clone
PK136
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08857AF350
Alexa Fluor 405
NBP3-08857AF405
Alexa Fluor 488
NBP3-08857AF488
Alexa Fluor 532
NBP3-08857AF532
Alexa Fluor 594
NBP3-08857AF594
Alexa Fluor 647
NBP3-08857AF647
Alexa Fluor 700
NBP3-08857AF700
Alexa Fluor 750
NBP3-08857AF750
Allophycocyanin
NBP3-08857APC
Biotin
NBP3-08857B
CoraFluor 1
NBP3-08857CL1
DyLight 350
NBP3-08857UV
DyLight 405
NBP3-08857V
DyLight 488
NBP3-08857G
DyLight 550
NBP3-08857R
DyLight 594
NBP3-08857DL594
DyLight 650
NBP3-08857C
DyLight 680
NBP3-08857FR
DyLight 755
NBP3-08857IR
FITC
NBP3-08857F
HRP
NBP3-08857H
Janelia Fluor 525
NBP3-08857JF525
Janelia Fluor 549
NBP3-08857JF549
Janelia Fluor 585
NBP3-08857JF585
Janelia Fluor 635
NBP3-08857JF635
Janelia Fluor 646
NBP3-08857JF646
Janelia Fluor 669
NBP3-08857JF669
PE
NBP3-08857PE
PerCP
NBP3-08857PCP
Unconjugated
NBP3-07532-100ug
mFluor Violet 450 SE
NBP3-08857MFV450
mFluor Violet 500 SE
NBP3-08857MFV500
mFluor Violet 610 SE
NBP3-08857MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07532-100ug
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CD161/NK1.1 Antibody (PK136) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07532).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
CD Mouse spleen and bone marrow cells enriched for NK-1+ cells
Localization
Cell Surface
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
Klrb1c
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml

Reactivity Notes

Does not react with Human.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD161/NK1.1 Antibody (PK136) - Azide and BSA Free

  • CD161 antigen
  • CD161
  • CD161/NK1.1
  • CD161c
  • CLEC5BC-type lectin domain family 5 member B
  • HNKR-P1a
  • killer cell lectin-like receptor subfamily B member 1
  • killer cell lectin-like receptor subfamily B, member 1
  • Klrb1c
  • Ly55c
  • Ly-59
  • Natural killer cell surface protein P1A
  • NKR-P1
  • NKR-P1AMGC138614
  • NKRP1ANKR

Background

NK-1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene, also known as CD161b/CD161c and Ly-55. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN- gamma production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. The PK136 antibody has been reported to deplete NK cells, induce redirected lysis, block NK cell function, and induce NK cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol Klrb1c