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CD133 Antibody (3F10) - BSA Free

Images

 
Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human esophageal cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated ...read more
Immunohistochemistry-Paraffin: CD133 Antibody (3F10) [NBP2-37741] - Analysis of human breast cancer tissues section using mouse anti-CD133 antibody (clone 3F10). The staining was developed using HRP-conjugated ...read more
Flow Cytometry: CD133 Antibody (3F10) [NBP2-37741] - Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).
ELISA: CD133 Antibody (3F10) [NBP2-37741] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Immunocytochemistry/ Immunofluorescence: CD133 Antibody (3F10) [NBP2-37741] - Upregulations of TIPRL, LC3 and CD133 in HCCs. Human tissues were stained with the indicated antibodies followed by confocal observation. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, Flow, ICC/IF, IHC
Clone
3F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

CD133 Antibody (3F10) - BSA Free Summary

Immunogen
Synthesized peptide of human CD133.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PROM1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30739275).
Theoretical MW
133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-37741 in the following applications:

Reactivity Notes

Use in Human reported in scientific literature (PMID:33814238).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CD133 Antibody (3F10) - BSA Free

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD133 Antibody (NBP2-37741)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD133 Antibody (NBP2-37741) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP2-37741

Research Areas for CD133 Antibody (NBP2-37741)

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1