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CD133 Antibody (2F8C5) - BSA Free

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Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against HEK293 (1) and PROM1-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against HEK293 (1) and PROM1-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis of Hela cells using PROM1 mouse mAb (green) and negative control (red).
Western Blot: CD133 Antibody (2F8C5) [NBP2-52473] - Analysis using PROM1 mAb against human PROM1 (AA: 20-108) recombinant protein. (Expected MW is 36.1 kDa)
ELISA: CD133 Antibody (2F8C5) [NBP2-52473] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
2F8C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52473-0.025mg
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CD133 Antibody (2F8C5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD133 (AA: 20-108) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PROM1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
97.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD133 Antibody (2F8C5) - BSA Free

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin 1 (CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD133 Antibody
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Applications: WB, ELISA, Flow, CyTOF-ready

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Bioinformatics

Gene Symbol PROM1