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CD11c Antibody (N418) [Unconjugated]

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Mouse splenocytes were stained with Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (F0120) and Rat Anti-Mouse MHC class II (I-A/I-E) APC-conjugated Monoclonal Antibody (FAB6118A). Quadrant markers were set based on normal hamster IgG control antibody staining.
Mouse splenocytes were stained with Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody (F0120) and Rat Anti-Mouse MHC class II ...read more
CD11c was detected in immersion fixed paraffin-embedded sections of mouse spleen using Hamster Anti-Mouse CD11c Monoclonal Antibody (Catalog # MAB69501) at 25 µg/ml overnight at 4 °C. Before incubation with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
N418
Clonality
Monoclonal
Host
Hamster
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB69501U-100UG
Alexa Fluor 405
FAB69501V-100UG
Alexa Fluor 488
FAB69501G-100
Alexa Fluor 532
FAB69501X
Alexa Fluor 594
FAB69501T-100UG
Alexa Fluor 647
FAB69501R-100UG
Alexa Fluor 700
FAB69501N-100UG
Alexa Fluor 750
FAB69501S-100UG
Allophycocyanin/Cy7
FAB69501APCCY7
Biotin
FAB69501B
CoraFluor 1
FAB69501CL1
DyLight 350
FAB69501D
DyLight 405
FAB69501E
DyLight 488
FAB69501K
DyLight 550
FAB69501L
DyLight 594
FAB69501M
DyLight 650
FAB69501W
DyLight 680
FAB69501Y
DyLight 755
FAB69501Z
FITC
FAB69501F
HRP
FAB69501H
Janelia Fluor 525
FAB69501JF525
Janelia Fluor 549
FAB69501I
Janelia Fluor 646
FAB69501J
Janelia Fluor 669
FAB69501JF669
PE/Atto594
FAB69501PEATT594
PE/Cy5.5
FAB69501PECY55
PE/Cy7
FAB69501PECY7
mFluor Violet 450 SE
FAB69501MFV450
mFluor Violet 500 SE
FAB69501MFV500
mFluor Violet 610 SE
FAB69501MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB69501-SP
Carrier Free
FAB69501G-100
Carrier Free
FAB69501V-100UG
Carrier Free
FAB69501U-100UG
Carrier Free
FAB69501T-100UG
Carrier Free
FAB69501S-100UG
Carrier Free
FAB69501R-100UG
Carrier Free
FAB69501N-100UG
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CD11c Antibody (N418) [Unconjugated] Summary

Immunogen
Mouse spleen dendritic cells
Marker
Dendritic Cell Marker
Specificity
Detects mouse CD11c.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Hamster
Gene
ITGAX
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 3-25 ug/mL
Publications
Read Publications using
MAB69501 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD11c Antibody (N418) [Unconjugated]

  • 95 alpha chain
  • 95
  • 95, alpha subunit
  • CD11 antigen-like family member C
  • CD11c antigen
  • CD11c
  • CD11Cleu M5, alpha subunit
  • Integrin alpha X
  • integrin alpha-X
  • integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
  • integrin, alpha X (complement component 3 receptor 4 subunit)
  • ITGAX
  • Leu M5
  • Leukocyte adhesion glycoprotein p150
  • Leukocyte adhesion receptor p150
  • leukocyte surface antigen p150
  • myeloid membrane antigen, alpha subunit
  • p150 95 integrin alpha chain
  • p150,95 alpha
  • SLEB6

Background

CD11c, also known as the Integrin alpha X subunit, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse CD11c extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat CD11c, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (Complement iC3b, ICAMs, vWF and Fibrinogen) while others (Osteopontin, Thy-1, Plasminogen, Heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.
  1. Metlay, J.P. et al. (1990) J. Exp. Med. 171:1753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11c Antibody (MAB69501)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: FACS, Flow Cytometry, IHC.


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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITGAX