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CD11b Antibody (238446) [Phycoerythrin]

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Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood monocytes were stained with Mouse Anti-Human Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P, filled histogram) or isotype control antibody (Catalog # IC0041P, open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
238446
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB16991U-100UG
Alexa Fluor 405
FAB16991V-100UG
Alexa Fluor 488
FAB16991G-100
Alexa Fluor 488
FAB16991G-025
Alexa Fluor 532
FAB16991X
Alexa Fluor 594
FAB16991T-100
Alexa Fluor 594
FAB16991T-025
Alexa Fluor 647
FAB16991R-100UG
Alexa Fluor 700
FAB16991N-100UG
Alexa Fluor 750
FAB16991S-100UG
Allophycocyanin/Cy7
FAB16991APCCY7
Biotin
FAB16991B
CoraFluor 1
FAB16991CL1
DyLight 350
FAB16991D
DyLight 405
FAB16991E
DyLight 488
FAB16991K
DyLight 550
FAB16991L
DyLight 594
FAB16991M
DyLight 650
FAB16991W
DyLight 680
FAB16991Y
DyLight 755
FAB16991Z
FITC
FAB16991F
HRP
FAB16991H
Janelia Fluor 525
FAB16991JF525
Janelia Fluor 549
FAB16991I
Janelia Fluor 585
FAB16991JF585
Janelia Fluor 635
FAB16991JF635
Janelia Fluor 646
FAB16991J
Janelia Fluor 669
FAB16991JF669
PE/Atto594
FAB16991PEATT594
PE/Cy5.5
FAB16991PECY55
PE/Cy7
FAB16991PECY7
Phycoerythrin
FAB16991P-025
Unconjugated
MAB16991
mFluor Violet 450 SE
FAB16991MFV450
mFluor Violet 500 SE
FAB16991MFV500
mFluor Violet 610 SE
FAB16991MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB16991
Carrier Free
MAB16991
Carrier Free
MAB16991-SP
Carrier Free
FAB16991T-100
Carrier Free
FAB16991T-025
Carrier Free
FAB16991P-025
Carrier Free
FAB16991G-100
Carrier Free
FAB16991G-025
Carrier Free
FAB16991U-100UG
Carrier Free
FAB16991N-100UG
Carrier Free
FAB16991S-100UG
Carrier Free
FAB16991R-100UG
Carrier Free
FAB16991V-100UG
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CD11b Antibody (238446) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha M/CD11b
Phe17-Asn1105
Accession # NP_001139280
Marker
Microglia Marker, Macrophage Marker
Specificity
Detects human Integrin alpha M/CD11b in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ITGAM
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB16991P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD11b Antibody (238446) [Phycoerythrin]

  • antigen CD11b (p170)
  • CD11 antigen-like family member B
  • CD11b antigen
  • CD11b
  • Cell surface glycoprotein MAC-1 subunit alpha
  • Complement component 3 receptor 3 subunit
  • CR-3 alpha chain
  • CR3A
  • CR3AMGC117044
  • Integrin alpha M
  • integrin alpha-M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • ITGAM
  • Leukocyte adhesion receptor MO1
  • MAC-1
  • MAC1A
  • macrophage antigen alpha polypeptide
  • MO1A
  • neutrophil adherence receptor alpha-M subunit
  • Neutrophil adherence receptor
  • SLEB6

Background

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and Complement Receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, Fibrinogen and the C3 Complement Fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD11b Antibody (FAB16991P)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(3)
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Human
(3)
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Showing Publications 1 - 6 of 6.
Publications using FAB16991P Applications Species
Chuchu Gong, Zhengyan Gu, Xinkang Zhang, Qingqiang Xu, Guanchao Mao, Zhipeng Pei et al. HMSCs exosome‐derived miR‐199a‐5p attenuates sulfur mustard‐associated oxidative stress via the CAV1/NRF2 signalling pathway Journal of Cellular and Molecular Medicine 8/1/2023 [PMID: 37386746]
Iris A. Otto, Paulina Nuñez Bernal, Margot Rikkers, Mattie H.P. van Rijen, Anneloes Mensinga, Moshe Kon et al. Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential iScience 9/16/2022 [PMID: 36105583]
Yasser Basmaeil, Abdullah Al Subayyil, Mohammad Abumaree, Tanvir Khatlani Conditions Mimicking the Cancer Microenvironment Modulate the Functional Outcome of Human Chorionic Villus Mesenchymal Stem/Stromal Cells in vitro Frontiers in Cell and Developmental Biology 6/21/2021 [PMID: 34235143]
S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial Biomed Res Int, 2020-07-27;2020(0):8748934. 2020-07-27 [PMID: 32802883] (Flow Cytometry, Human) Flow Cytometry Human
Li M, Piao L, Chen C, Wu X, Yeh C, Masch R, Chang C, Huang S Modulation of Decidual Macrophage Polarization by Macrophage Colony-Stimulating Factor Derived from First-Trimester Decidual Cells: Implication in Preeclampsia. Am J Pathol, 2016-03-10;186(5):1258-66. 2016-03-10 [PMID: 26970370] (Flow Cytometry, Human) Flow Cytometry Human
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008-06-30;84(4):1109-19. 2008-06-30 [PMID: 18591415] (Flow Cytometry, Human) Flow Cytometry Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD11b Antibody (FAB16991P). (Showing 1 - 9 of 9 FAQs).

  1. I am looking for a Mac-1 (microglia cell marker) antibody to do immunostaining with my mouse brain and I checked, you have a lot of antibodies for this biomarker. I don't know which one is the best to be used for immunohistology on mouse brain tissue. Could you please help with this?
    • I would recommend NB110-89474. This antibody has been published in 11 scientific articles, including several times in IHC in mouse. As long as CD11b is expressed in your sample, it should work just fine and is backed by our 100% Novus Guarantee.
  2. What types of cells can this CD11b antibody be used as a marker for?
    • A study used this CD11b antibody to stain granulocytes in mouse uterine tissue in IHC. CD11b is also expressed on other leukocytes such as monocytes, macrophages, and natural killer cells.
  3. Do you know if this CD11b antibody can be used in CD11b positive cell depletion? 
    • This particular CD11b antibody is not reccomended for depletion studies due to the preservative used; however, our CD11b antibody NBP1-06650 is offered in a preservative free format and has been validated for blocking.
  4. Looking for a bone marrow macrophage marker can you recommend a CD11b antibody for ICC?
    • I would recommend NB110-89474 which is our most popular CD11b antibody and has been validated in ICC and cited in 18 publications.
  5. We need a CD11b antibody for IHC-P on mouse tissue conjugated to FITC, do you have one that will work?
    • We have 3 CD11b antibodies for IHC-P and just one CD11 b antibody we offer conjugated to FITC, NB110-89474F.
  6. The protocol says to do enzymatic antigen retrieval before incubating with the CD11b antibody, which enzyme is used?
    • The protocol is referencting the PIER method using Proteinase K as the enzyme.
  7. I'm not sure if this will work for me, can I get a free sample of your CD11b antibody to test?
    • We don't offer free samples but we do offer this CD11b antibody in a trial size of 0.025 ml at a reduced cost, all antibodies are backed by our 100% guarantee to work in all validated species and applications on the product's web page.
  8. Is CD11b antibody also staining neutrophils in the brain
    • CD11b is typically used as a marker for granulocytes and macrophages. You may need a combination of markers to specifically identify neutrophils
  9. What is the PE:Ab molar ratio for this antibody? (lot # lns0312091)
    • We do not specifically test the post-conjugation ratio of PE to the antibody, but we expect the ratio to be 1:1 based on production techniques.

Secondary Antibodies

 

Isotype Controls

Additional CD11b Products

Blogs on CD11b.

Check out the latest blog posts on CD11b.

  Read full blog post.
Antibody treatment can generate microglia-like cells from bone marrow
By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys...  Read full blog post.
TMEM 119 is a specific marker of microglia cells
By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra...  Read full blog post.
Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.
CD11b - More than a microglial marker
The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the...  Read full blog post.
CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.
Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases
Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This...  Read full blog post.
CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies
The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a...  Read full blog post.
The CD11b Antibody: A Marker for Microglial Cells
Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac...  Read full blog post.
CD11b Expression, Leukocyte Adhesion and the Innate Immune System
What is CD11b?CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as o...  Read full blog post.
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Bioinformatics

Gene Symbol ITGAM
Uniprot