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POLD3 Antibody (3E2) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01] - Analysis of monoclonal antibody to POLD3 on HeLa cell . Antibody concentration 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01] - Analysis of monoclonal antibody to POLD3 on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: POLD3 Antibody (3E2) [H00010714-M01] - Detection limit for recombinant GST tagged POLD3 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
3E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

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POLD3 Antibody (3E2) - Azide and BSA Free Summary

Immunogen
POLD3 (NP_006582, 357 a.a. ~ 466 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPLEPVPKTEPEPPSVKSSSGENKRKRKRVLKSKTYLDGEGCIVTEKVYESESCTDSEEELNMKTSSVHRPPAMTVKKEPREERKGPKKGTAALGKANRQVSITGFFQRK
Specificity
POLD3 - polymerase (DNA-directed), delta 3, accessory subunit
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POLD3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:500
Publications
Read Publications using
H00010714-M01 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLD3 Antibody (3E2) - Azide and BSA Free

  • DNA polymerase delta subunit 3
  • DNA polymerase delta subunit p66
  • DNA polymerase delta, subunit 3
  • KIAA0039MGC119643
  • MGC119642
  • P66
  • P68
  • polymerase (DNA directed), delta 3
  • polymerase (DNA-directed), delta 3, accessory subunit

Background

The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA; MIM 176740), the multisubunit replication factor C (see MIM 102579), and the 4 subunit polymerase complex: POLD1 (MIM 174761), POLD2 (MIM 600815), POLD3, and POLD4 (MIM 611525) (Liu and Warbrick, 2006 [PubMed 16934752]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POLD3 Antibody (H00010714-M01)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Human
(2)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using H00010714-M01 Applications Species
Lu R, Nelson CB, Rogers S et Al. Distinct modes of telomere synthesis and extension contribute to Alternative Lengthening of Telomeres iScience 2023-12-11 [PMID: 38213617]
Rosso I, Jones-Weinert C, Rossiello F et al. Alternative lengthening of telomeres (ALT) cells viability is dependent on C-rich telomeric RNAs Nature communications 2023-11-04 [PMID: 37925537]
Rose AM, Goncalves T, Cunniffe S et al. Induction of the alternative lengthening of telomeres pathway by trapping of proteins on DNA Nucleic acids research 2023-03-20 [PMID: 36940725] (WB, Human)

Details:
Dilution used in WB 1:500 		WB Human
Madhura D, Theodore P, Nahed J et al. Error-prone repair of stalled replication forks drives mutagenesis and loss of heterozygosity in haploinsufficient BRCA1 cells. Mol Cell. 2022-09-07 [PMID: 36099913]
Shixin C, John W, Nicole B et al. Cockayne syndrome group B protein uses its DNA translocase activity to promote mitotic DNA synthesis DNA Repair (Amst). 2022-06-17 [PMID: 35738143]
Camelia M, Eleftheria K, Maria F et al. DNA replication is highly resilient and persistent under the challenge of mild replication stress. Cell Rep. 2022-04-19 [PMID: 35443178]
Barroso-GonzAlez J, GarcIa-ExpOsito L, Galaviz P et al. Anti-recombination function of MutS alpha restricts telomere extension by ALT-associated homology-directed repair Cell reports 2021-12-07 [PMID: 34879271]
Silva B, Arora R, Bione S, Azzalin CM. TERRA transcription destabilizes telomere integrity to initiate break-induced replication in human ALT cells Nature Communications 2021-06-18 [PMID: 34145295]
Raghunandan M, Geelen D, Majerova E, Decottignies A NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres The EMBO journal 2021-02-17 [PMID: 33595114]
Zhang H, Zhao R, Tones J et al. Nuclear body phase separation drives telomere clustering in ALT cancer cells Mol. Biol. Cell 2020-06-24 [PMID: 32579423]
Show All 15 Publications.

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Bioinformatics

Gene Symbol POLD3
Entrez
Uniprot