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CCL8/MCP-2 Antibody [Biotin]

 (4 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF-281-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-281-NA
Carrier Free
AF-281-NA
Carrier Free
AF-281-SP
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CCL8/MCP-2 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL8/MCP-2 (R&D Systems, Catalog # 281-CP)
Gln24-Pro99
Accession # P80075
Specificity
Detects human CCL8/MCP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, recombinant mouse (rm) MIP-1 alpha , rhMCP-1, and rhMCP-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL8
Purity
Antigen Affinity-purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL8/MCP-2 Antibody (Catalog # MAB281)
ELISA Detection: Human CCL8/MCP-2 Biotinylated Antibody (Catalog # BAF281)
Standard: Recombinant Human CCL8/MCP-2 (Catalog # 281-CP)
Readout System
Publications
Read Publications using
BAF281 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Antigen Affinity-purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL8/MCP-2 Antibody [Biotin]

  • C-C motif chemokine 8
  • CCL8
  • chemokine (C-C motif) ligand 8
  • HC14
  • MCP2
  • MCP-2
  • member 8 (monocyte chemotactic protein 2)
  • monocyte chemoattractant protein 2
  • SCYA10
  • small-inducible cytokine A8

Background

MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.

Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL8/MCP-2 Antibody (BAF281)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, ELISA Development (Detection).


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ELISA Development
(2)
ELISA Development (Detection)
(2)
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(4)
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Bioinformatics

Gene Symbol CCL8
Uniprot