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CCL7/MCP-3/MARC Antibody

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Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate
Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Jurkat cell lysate
Immunohistochemistry-Frozen: CCL7/MCP-3/MARC Antibody [NBP1-82367] - CCL7 (green) stained frozen human skin section, counterstained with DAPI (40x). Sections fixed with 4% PFA PBS, permeabilized with 0.5% Triton TBS ...read more
Western Blot: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Fetal Small Intestine.
Immunohistochemistry: CCL7/MCP-3/MARC Antibody [NBP1-82367] - Human Heart Cellular data: Myocardial cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CCL7/MCP-3/MARC Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptide towards MCP3. Peptide sequence TSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPT. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCL7
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml
Application Notes
Validated for use in IHC-F reported by a verified customer review.
Theoretical MW
9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-82367 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for CCL7/MCP-3/MARC Antibody

  • C-C motif chemokine 7
  • CCL7
  • chemokine (C-C motif) ligand 7
  • FIC
  • MARC
  • MCP-3
  • MCP3Monocyte chemotactic protein 3
  • MCP-3Small-inducible cytokine A7
  • MGC138465
  • Monocyte chemoattractant protein 3
  • NC28SCYA6
  • SCYA7MGC138463
  • small inducible cytokine A7 (monocyte chemotactic protein 3)

Background

MCP3 encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL7/MCP-3/MARC Antibody (NBP1-82367) (0)

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Review for CCL7/MCP-3/MARC Antibody (NBP1-82367) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-82367:
Filter by Applications
IHC-Fr
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CCL7/MCP-3/MARC NBP1-82367
Enlarge 		5
reviewed by:
Tyler Crowe
 IHC-Fr Human 01/18/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotQC25545-41880

Comments

CommentsSections fixed with 4% PFA PBS, permeabilized with 0.5% Triton TBS Ca/Azide, and blocked with 5% BSA TBS Ca/Azide at 37C for 1 hour. Antibody diluted 1:50 in block, and incubated for 1 hour at 37C. Sections were washed and stained with secondary antibody, and counterstained with DAPI.

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Secondary Antibodies

 

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Recent Reviews

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1
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Immunohistochemistry-Frozen CCL7/MCP-3/MARC NBP1-82367
Enlarge
 Tyler Crowe
01/18/2019
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol CCL7
Uniprot