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CCL22/MDC Antibody [Unconjugated]

 (12 Publications)
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Recombinant Mouse CCL22/MDC (Catalog # 439‑MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.
Recombinant Mouse CCL22/MDC (Catalog # 439‑MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF439
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF439-SP
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CCL22/MDC Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL22/MDC
Gly25-Ser92
Accession # Q9QZU2
Specificity
Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL22
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Neutralization 0.3-1.0 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF439 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody [Unconjugated]

  • A-152E5.1
  • ABCD-1
  • CC chemokine STCP-1
  • C-C motif chemokine 22
  • CCL22
  • chemokine (C-C motif) ligand 22
  • DC/B-CK
  • Macrophage-derived chemokine
  • MDC
  • MDCStimulated T-cell chemotactic protein 1
  • MGC34554
  • SCYA22MDC(1-69)
  • small inducible cytokine A22
  • small inducible cytokine subfamily A (Cys-Cys), member 22
  • Small-inducible cytokine A22
  • STCP-1
  • stimulated T cell chemotactic protein 1

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL22/MDC Antibody
[Unconjugated]
AF439
CCL22/MDC Antibody [Unconjugated]
Species: Mu
Applications: WB, B/N

Publications for CCL22/MDC Antibody (AF439)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, Functional Assay, In Vivo, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
Functional Assay
(1)
In Vivo
(1)
Neutralization
(6)
Western Blot
(1)
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Filter By Species
Mouse
(10)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF439 Applications Species
Yu Li, Pablo Guaman Tipan, Hilary J Selden, Jayashree Srinivasan, Laura P Hale, Lauren I R Ehrlich CCR4 and CCR7 differentially regulate thymocyte localization with distinct outcomes for central tolerance eLife 6/2/2023 [PMID: 37266571]
D Thumkeo, S Punyawatth, S Prasongtan, R Matsuura, K Arima, H Nie, R Yamamoto, N Aoyama, H Hamaguchi, S Sugahara, S Takeda, V Charoensaw, A Tanaka, S Sakaguchi, S Narumiya PGE2-EP2/EP4 signaling elicits immunosuppression by driving the mregDC-Treg axis in inflammatory tumor microenvironment Cell Reports, 2022-06-07;39(10):110914. 2022-06-07 [PMID: 35675777] (In Vivo, Mouse) In Vivo Mouse
KE Chen, M Ghosh, L Rivera, S Lin, A Kumar, S Swaminatha, MY Lorenson, AM Walker Prolactin enhances T regulatory cell promotion of breast cancer through the long form prolactin receptor Translational Oncology, 2021-08-07;14(11):101195. 2021-08-07 [PMID: 34375938] (Neutralization, Mouse) Neutralization Mouse
Jianlin Zhang, Kevin P. Vincent, Angela K. Peter, Matthew Klos, Hongqiang Cheng, Selina Manying Huang et al. Cardiomyocyte Expression of ZO-1 Is Essential for Normal Atrioventricular Conduction but Does Not Alter Ventricular Function Circulation Research 7/3/2020 [PMID: 32345129]
S Yi, J Zhai, R Niu, G Zhu, M Wang, J Liu, H Huang, Y Wang, X Jing, L Kang, W Song, Y Shi, H Tang Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge Nat Commun, 2018-09-24;9(1):3879. 2018-09-24 [PMID: 30250029] (Neutralization, Mouse) Neutralization Mouse
Araujo-Pires A, Vieira A, Francisconi C, Biguetti C, Glowacki A, Yoshizawa S, Campanelli A, Trombone A, Sfeir C, Little S, Garlet G IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis. J Bone Miner Res, 2015-03-01;30(3):412-22. 2015-03-01 [PMID: 25264308] (Neutralization, Mouse) Neutralization Mouse
Loebbermann , Jens, Durant , Lydia, Thornton , Hannah, Johansson , Cecilia, Openshaw , Peter J Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells. Proc Natl Acad Sci U S A, 2013-02-04;110(8):2987-92. 2013-02-04 [PMID: 23382205] (Neutralization, Mouse) Neutralization Mouse
Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets. J. Clin. Invest., 2011-08-01;121(0):3024. 2011-08-01 [PMID: 21737880] (Western Blot, Mouse) Western Blot Mouse
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. J. Exp. Med., 2008-03-03;205(3):699-710. 2008-03-03 [PMID: 18316417] (Neutralization, Mouse) Neutralization Mouse
Show All 12 Publications.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCL22
Uniprot