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CCL22/MDC Antibody [Biotin]

 (3 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF439
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF439
Carrier Free
AF439
Carrier Free
AF439-SP
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CCL22/MDC Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse CCL22/MDC (R&D Systems, Catalog # 439-MD)
Gly25-Ser92
Accession # Q9QZU2
Specificity
Detects mouse MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity recombinant human (rh) MDC, recombinant mouse (rm) 6Ckine, rmFractalkine, and rmTARC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL22
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse CCL22/MDC Antibody (Catalog # MAB4391)
ELISA Detection: Mouse CCL22/MDC Biotinylated Antibody (Catalog # BAF439)
Standard: Recombinant Mouse CCL22/MDC (Catalog # 439-MD)
Readout System
Publications
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BAF439 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL22/MDC Antibody [Biotin]

  • A-152E5.1
  • ABCD-1
  • CC chemokine STCP-1
  • C-C motif chemokine 22
  • CCL22
  • chemokine (C-C motif) ligand 22
  • DC/B-CK
  • Macrophage-derived chemokine
  • MDC
  • MDCStimulated T-cell chemotactic protein 1
  • MGC34554
  • SCYA22MDC(1-69)
  • small inducible cytokine A22
  • small inducible cytokine subfamily A (Cys-Cys), member 22
  • Small-inducible cytokine A22
  • STCP-1
  • stimulated T cell chemotactic protein 1

Background

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca2+ mobilization in activated mouse and human T cells.

  1. Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
  2. Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
  3. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
  4. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL22/MDC Antibody
[Biotin]
BAF439
CCL22/MDC Antibody [Biotin]
Species: Mu
Applications: WB, ELISA(Det)

Publications for CCL22/MDC Antibody (BAF439)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ELISA Development, IHC-P, Luminex Development.


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ELISA Development
(1)
IHC-P
(1)
Luminex Development
(1)
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(3)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCL22
Uniprot