![Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Western-Blot-NBP2-80618-img0002.jpg "Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6) cell lysates. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, Rt, Fe, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 2D8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Full lenght human MCP1 expressed in E. coli. [Uniprot: P13500] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CCL2 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 11 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
![Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Block_Neutralize_9064.jpg)
![Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/CXCL8_MAB208_Immunocytochemistry_9339.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](https://images.novusbio.com/images2/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-1882021143847.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CCL2/MCP1 Antibody (NBP2-80618)Find related products by research area.
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MCP-1 - chemoattractant protein involed in monocyte migration and infiltration Monocyte chemotractant protein-1 (MCP-1), also known as CCL2, is a key chemokine involved in the migration of monocytes and macrophages to sites of active inflammation. It is a member of the C-C/beta family of cytokines, characterized by the Cys-C... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CCL2 |
![Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated (vehicle control, e), 1uM lapatinib (f) or 1 uM IKKi compound A (g) for 24 hrs. Bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02481-5), licensed under a CC-BY license. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80618-img0004.jpg "Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - CCL2 staining in acini cultures from MMTV-HER2 MGs (20-24 weeks) grown for 5 days and then DMSO-treated (vehicle control, e), 1uM lapatinib (f) or 1 uM IKKi compound A (g) for 24 hrs. Bar: 25 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-02481-5), licensed under a CC-BY license. Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80618-img0006.jpg "Immunohistochemistry-Paraffin: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of paraffin-embedded liver cancer tissues using MCP1 mouse mAb with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80618-img0007.jpg "Flow Cytometry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of A549 cells using MCP1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.")
![Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa) Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Western-Blot-NBP2-80618-img0001.jpg "Western Blot: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis using MCP1 mAb against human MCP1 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa) Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). B) Staining without MCP1 mAb Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80618-img0003.jpg "Immunocytochemistry/Immunofluorescence: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Analysis of HepG2 cells using A) MCP1 mAb (green), DRAQ5 fluorescent DNA dye (blue), and Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red). B) Staining without MCP1 mAb Image from the standard format of this antibody.")
![Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, CCR2 signal, zoom factor 1x; full image in Supplementary Fig. 5i. CCL2 intensity was quantified usin](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80618-img0005.jpg "Immunohistochemistry: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - HER2 activates NF-kB and upregulates CCL2. MGs from FVB wild-type and 22-week-old MMTV-HER2 mice stained against CCL2, HER2, and CCR2. Bars: 25 um. Inset, CCR2 signal, zoom factor 1x; full image in Supplementary Fig. 5i. CCL2 intensity was quantified usin")
![ELISA: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-2D8-Azide-and-BSA-Free-ELISA-NBP2-80618-img0008.jpg "ELISA: CCL2/MCP1 Antibody (2D8) - Azide and BSA Free [NBP2-80618] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng). Image from the standard format of this antibody.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/CCL5_DRN00_ELISA_393.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/DATA_CCL5_DRN00_ELISA_810.jpg)
![N/A CCL5/RANTES [HRP]](https://images.novusbio.com/images2/DATA_CCL5_DRN00_ELISA_811.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Immunohistochemistry CCR2 Antibody (48607) [Unconjugated]](https://images.novusbio.com/images2/CCR2_MAB150_Immunohistochemistry_18879.jpg)
![Flow Cytometry CCR2 Antibody (48607) [Unconjugated]](https://images.novusbio.com/images2/CCR2_MAB150_Flow_Cytometry_19981.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_723.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/DATA_CXCL10_DIP100_ELISA_724.jpg)
![N/A CXCL10/IP-10/CRG-2 [HRP]](https://images.novusbio.com/images2/CXCL10_DIP100_ELISA_121.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images2/ICAM1_AF796_Immunohistochemistry_17354.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241048112.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](https://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481112.jpg)
![N/A CD46 Antibody (344519) [Unconjugated]](https://images.novusbio.com/images/antibody/mab2005_human-cd46-mab-clone-344519-209202413163..jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_304.jpg)
![Bioactivity IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_306.jpg)
![SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images/201-lb_recombinant-human-il-1-beta-il-1f2-protein-sec-mals-123202491556.jpg)
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")