Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 75 amino acids corresponding to CCL13/MCP-4 Source: E. coli Uniprot ID: Q99616 Amino Acid Sequence: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT |
Details of Functionality | MCP4 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | CCL13 |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of CCL13/MCP-4 as determined by LAL method. |
Dilutions |
|
Theoretical MW | 8.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for CCL13/MCP-4 Protein (NBP2-34973)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.