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Recombinant Mouse CCL11/Eotaxin Protein

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SDS-Page: Mouse, CCL11/Eotaxin Protein [NBP2-35177]
SDS-Page: Mouse, CCL11/Eotaxin Protein [NBP2-35177]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35177-1mg
Carrier-Free
NBP2-35177-100ug
Carrier-Free
NBP2-35177-500ug
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Recombinant Mouse CCL11/Eotaxin Protein Summary

Description
A single non-glycosylated polypeptide chain containing 74 amino acids corresponding to CCL11/Eotaxin Source: E. coli

Uniprot ID: P48298

Amino Acid Sequence: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Details of Functionality
Eotaxin Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CCL11
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of CCL11/Eotaxin as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Mouse CCL11/Eotaxin Protein

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • Eotaxin/CCL11
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CCL11
Uniprot