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CCL11/Eotaxin Antibody (42285) [Unconjugated]

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Recombinant Mouse CCL11/Eotaxin (Catalog # 420‑ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by MTT staining. Chemotaxis elicited by Recombinant Mouse CCL11/Eotaxin (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420). The ND50 is typically 3‑30 µg/mL.
Recombinant Mouse CCL11/Eotaxin (Catalog # 420‑ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more
CCL11/Eotaxin was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clone
42285
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAM420
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB420-SP
Carrier Free
MAB420-500
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CCL11/Eotaxin Antibody (42285) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL11/Eotaxin
His24-Pro97
Accession # P48298
Specificity
Detects mouse CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CCL11/Eotaxin, recombinant mouse CCL7, and rhCCL21 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CCL11
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 3-30 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB420 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL11/Eotaxin Antibody (42285) [Unconjugated]

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the amino-terminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma . In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.

  1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
  2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
  3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL11/Eotaxin Antibody (MAB420)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Neutralization, Western Blot.


Filter By Application
Neutralization
(6)
Western Blot
(1)
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Mouse
(6)
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Showing Publications 1 - 8 of 8.
Publications using MAB420 Applications Species
G Scabia, G Testa, M Scali, S Del Turco, G Desiato, N Berardi, A Sale, M Matteoli, L Maffei, M Maffei, M Mainardi, “Train the Reduced ccl11/eotaxin mediates the beneficial effects of environmental stimulation on the aged hippocampus Brain, Behavior, and Immunity, 2021-08-19;98(0):234-244. 2021-08-19 [PMID: 34418501] (Neutralization, Western Blot, Mouse) Neutralization, Western Blot Mouse
Tawfik Khoury, Dory Rotnemer-Golinkin, Lidya Zolotarev, Yaron Ilan Orally administered anti-eotaxin-1 monoclonal antibody is biologically active in the gut and alleviates immune-mediated hepatitis: A novel anti-inflammatory personalized therapeutic approach Int J Immunopathol Pharmacol 2021-07-19 [PMID: 34275345]
Kristen L. Mittelsteadt, Erika T. Hayes, Daniel J. Campbell ICOS signaling limits regulatory T cell accumulation and function in visceral adipose tissue Journal of Experimental Medicine 2021-06-07 [PMID: 33881452]
F Wang, N Baba, Y Shen, T Yamashita, E Tsuru, M Tsuda, N Maeda, Y Sagara CCL11 promotes migration and proliferation of mouse neural progenitor cells Stem Cell Res Ther, 2017-02-07;8(1):26. 2017-02-07 [PMID: 28173860] (Neutralization, Mouse) Neutralization Mouse
Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson&#039;s disease J Biol Chem, 2016-05-12;0(0):. 2016-05-12 [PMID: 27226559] (Neutralization, Mouse) Neutralization Mouse
Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Nature, 2011-08-31;477(7362):90-4. 2011-08-31 [PMID: 21886162] (Neutralization, Mouse) Neutralization Mouse
Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity. Clin. Exp. Allergy, 2011-07-04;41(10):1447-55. 2011-07-04 [PMID: 21722219] (Neutralization, Mouse) Neutralization Mouse
Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice. Am. J. Respir. Crit. Care Med., 2008-09-11;178(10):1023-32. 2008-09-11 [PMID: 18787221] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol CCL11
Uniprot