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CCL1/I-309/TCA-3 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF272
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF272
Carrier Free
AF272
Carrier Free
AF272-SP
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CCL1/I-309/TCA-3 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human CCL1/I‑309/TCA‑3 (R&D Systems, Catalog # 272-I)
Lys24-Lys96
Accession # P22362
Specificity
Detects human CCL1/I‑309/TCA‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with rhMIP-1 beta , rvMIP-1, rrIFN-gamma , rmTCA-3 and rhTARC is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human CCL1/I-309/TCA-3 Antibody (Catalog # MAB272)
ELISA Detection: Human CCL1/I-309/TCA-3 Biotinylated Antibody (Catalog # BAF272)
Standard: Recombinant Human CCL1/I-309/TCA-3 (Catalog # 272-I)
Readout System
Publications
Read Publications using
BAF272 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL1/I-309/TCA-3 Antibody [Biotin]

  • C-C motif chemokine 1
  • CCL1
  • chemokine (C-C motif) ligand 1
  • I-309
  • inflammatory cytokine I-309
  • P500
  • SCYA1
  • SCYA1Small-inducible cytokine A1
  • SCYA2
  • SISe
  • small inducible cytokine A1 (I-309, homologous to mouse Tca-3)
  • T lymphocyte-secreted protein I-309
  • TCA3
  • TCA-3

Background

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15-16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL1/I-309/TCA-3 Antibody (BAF272)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA Development.


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(2)
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Bioinformatics

Gene Symbol CCL1
Uniprot