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Rat Cathepsin L ELISA Kit (Colorimetric)

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ELISA: Rat Cathepsin L ELISA Kit (Colorimetric) [NBP2-89173] - Samples were spiked with high concentrations of Rat Cathepsin L and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Cathepsin L ELISA Kit (Colorimetric) [NBP2-89173] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.63-40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

Rat Cathepsin L ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CTSL in samples. No Significant cross-reactivity or interference between Rat CTSL and analogues was observed.
Standard Curve Range
0.63-40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 15.77
Intra-Assay
CV% < 7.35
Spike Recovery
88-107%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CTSL

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Cathepsin L ELISA Kit (Colorimetric)

  • Cathepsin L
  • cathepsin L1
  • CATL
  • CTSL
  • CTSL1
  • CTSLEC 3.4.22.15
  • EC 3.4.22
  • FLJ31037
  • Major excreted protein
  • MEP

Background

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CTSL