![Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NBP2-41181-img0001.jpg "Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ELISA, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK. Amino Acid Squence: YPIPHTTRPPKKDEENGKN |
| Specificity | At least six alternatively spliced transcript variants have been observed. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CASK |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Simple Western reported in a verified customer review. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
| Publications using NBP2-41181 | Applications | Species |
|---|---|---|
| Becker M, Mastropasqua F, Reising JP et al. Presynaptic dysfunction in CASK-related neurodevelopmental disorders Transl Psychiatry 2020-09-14 [PMID: 32929080] (ICC/IF, Simple Western, WB, Human) | ICC/IF, Simple Western, WB | Human |
| Li D Genetic implications of individual intervention and neuronal dysfunction in neurodevelopmental disorders Thesis 2022-01-01 (ICC/IF, WB, Human) | ICC/IF, WB | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Martin Becker |
Simple Western | Human | 03/13/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for CASK Antibody (NBP2-41181)Find related products by research area.
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| Gene Symbol | CASK |
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41181-img0002.jpg "Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.")
![Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.](http://images.novusbio.com/fullsize/CASK-Antibody-Simple-Western-NBP2-41181-img0003.jpg "Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.")
![Simple Western: CASK Antibody - BSA Free [NBP2-41181] - Presynaptic effect is limited to inhibitory VGAT presynaptic marker.a Deconvolution of cell-type populations underlying bulk RNA-sequence samples from all cell lines. b Quantification of VGlut & Homer-1 particle size (n = 4 per cell line). c Representative confocal microscopy images of neurons differentiated for 28 days & immunostained for inhibitory VGAT (green), Homer-1 (red), & Hoechst (blue). Scale = 10 µm. d Quantification of VGAT & Homer-1 particle size (n = 3 per cell line). Statistical differences between cell lines were calculated using ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01, ***p < 0.001. Asterisks are color-coded according to case cell lines. e In vivo concentration of GABA in the DLPFC, MFC, & putamen of ASDCASK_SS & his cotwin in relation to a typical developed control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32929080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-41181_rabbit-polyclonal-cask-antibody-31020241612227.jpg "Simple Western: CASK Antibody - BSA Free [NBP2-41181] - Presynaptic effect is limited to inhibitory VGAT presynaptic marker.a Deconvolution of cell-type populations underlying bulk RNA-sequence samples from all cell lines. b Quantification of VGlut & Homer-1 particle size (n = 4 per cell line). c Representative confocal microscopy images of neurons differentiated for 28 days & immunostained for inhibitory VGAT (green), Homer-1 (red), & Hoechst (blue). Scale = 10 µm. d Quantification of VGAT & Homer-1 particle size (n = 3 per cell line). Statistical differences between cell lines were calculated using ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01, ***p < 0.001. Asterisks are color-coded according to case cell lines. e In vivo concentration of GABA in the DLPFC, MFC, & putamen of ASDCASK_SS & his cotwin in relation to a typical developed control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32929080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
