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CARS Antibody (CL2309)

Images

 
Immunohistochemistry: CARS Antibody (CL2309) [NBP2-36757] - Staining of human breast cancer shows cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry: CARS Antibody (CL2309) [NBP2-36757] - Staining of human skin shows strong cytoplasmic positivity in epidermis cells.
Immunohistochemistry: CARS Antibody (CL2309) [NBP2-36757] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in glandular epithelium cells.
Immunohistochemistry: CARS Antibody (CL2309) [NBP2-36757] - Staining of human colon shows cytoplasmic immunoreactivity in both epithelial and lamina propria cells.
Immunohistochemistry: CARS Antibody (CL2309) [NBP2-36757] - Staining of human testis shows strong cytoplasmic positivity in seminiferous tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
CL2309
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CARS Antibody (CL2309) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:SQCNLYMAARKAVRKRPNQALLENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLSEFREGVRKIAREQKVPEILQLSDALRDNILPELGVRFEDHEGLPTVVKLVDRNTLLKEREEKRRVE
Epitope
EDSSLGFPVG
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CARS1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500-1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (86%), Rat (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CARS Antibody (CL2309)

  • CARS1
  • CysRS
  • cysteine translase
  • cysteine tRNA ligase 1, cytoplasmic
  • Cysteine--tRNA ligase
  • cysteinyl-tRNA synthetase
  • cysteinyl-tRNA synthetase, cytoplasmic
  • EC 6.1.1
  • EC 6.1.1.16
  • MGC:11246

Background

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CARS1
Uniprot