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CARD9 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Analysis in human spleen and cerebral cortex tissues. Corresponding CARD9 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human spleen shows weak to moderate cytoplasmic positivity in a subset of leukocytes.
Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human tonsil shows moderate cytoplasmic positivity in a subset of leukocytes.
Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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CARD9 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: GSPKQPFAALHQEQVLRNPHDAGLSSGEPPEKERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARD9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CARD9 Protein (NBP2-37975PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CARD9 Antibody

  • CANDF2
  • CARD9
  • caspase recruitment domain family, member 9
  • caspase recruitment domain-containing protein 9
  • hCARD9

Background

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10. CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kB. CARD9 is an upstream activator of BCL-10 and NF-kB signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CARD9
Uniprot