CARD9 Antibodies
We offer CARD9 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each CARD9 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CARD9 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CARD9 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CARD9 polyclonal antibodies and browse our CARD9 monoclonal antibody catalog.
Alternate Names for CARD9 Antibodies
anti-CARD9 antibody, anti-CANDF2 antibody, anti-caspase recruitment domain family, member 9 antibody, anti-caspase recruitment domain-containing protein 9 antibody, anti-hCARD9 antibody
8 Results for "card9" in Primary Antibodies
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Analysis in human spleen and cerebral cortex tissues. Corresponding CARD9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Analysis in human spleen and cerebral cortex tissues. Corresponding CARD9 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human spleen shows weak to moderate cytoplasmic positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0009.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0006.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human tonsil shows moderate cytoplasmic positivity in a subset of leukocytes.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0007.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0008.jpg)
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP2-37975] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP2-37975-img0010.jpg)
![Western Blot: CARD9 Antibody (PSH0-41) [NBP3-32106] - Western blot analysis of CARD9 on different lysates with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 62.2 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32106_rabbit-card9-mab-psh0-41-278202416285236.jpg "Western Blot: CARD9 Antibody (PSH0-41) [NBP3-32106] - Western blot analysis of CARD9 on different lysates with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/1,000 dilution. Lane 1: HL-60 cell lysate Lane 2: THP-1 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 62.2 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: CARD9 Antibody (PSH0-41) [NBP3-32106] - Immunocytochemistry analysis of THP-1 cells labeling CARD9 with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CARD9 antibody (NBP3-32106) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32106_rabbit-card9-mab-psh0-41-29202410334029.jpg)
![Flow Cytometry: CARD9 Antibody (PSH0-41) [NBP3-32106] - Flow cytometric analysis of THP-1 cells labeling CARD9. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32106, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32106_rabbit-card9-mab-psh0-41-29202416372116.jpg)
![Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-32982-img0004.jpg "Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CARD9 Antibody [NBP1-32982] - H1299 xenograft, using CARD9 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunohistochemistry-Paraffin-NBP1-32982-img0002.jpg)
![Western Blot: CARD9 Antibody [NBP1-32982] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-32982-img0003.jpg)
![Western Blot: CARD9 Antibody [NBP1-76679] - Analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-NBP1-76679-img0004.jpg "Western Blot: CARD9 Antibody [NBP1-76679] - Analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 antibody at 2.5 ug /ml.")
![Immunocytochemistry/ Immunofluorescence: CARD9 Antibody - BSA Free [NBP1-76679] - Immunocytochemistry of CARD9 in K562 cells with CARD9 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76679_rabbit-polyclonal-card9-antibody-271120242042575.jpg)
![Western Blot: CARD9 Antibody [H00064170-B01P] - Analysis of CARD9 expression in transfected 293T cell line by CARD9 polyclonal antibody. Lane 1: CARD9 transfected lysate(54.12 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CARD9-Antibody-Western-Blot-H00064170-B01P-img0002.jpg "Western Blot: CARD9 Antibody [H00064170-B01P] - Analysis of CARD9 expression in transfected 293T cell line by CARD9 polyclonal antibody. Lane 1: CARD9 transfected lysate(54.12 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: CARD9 Antibody [H00064170-B01P] - Analysis of purified antibody to CARD9 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/CARD9-Antibody-Immunocytochemistry-Immunofluorescence-H00064170-B01P-img0001.jpg)
![Immunoprecipitation:CARD9 AntibodyNBP3-30275] -Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (NBP3-30275 lot 2) used for IP at 1 µg per reaction. CARD9 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30275-1). For blotting immunoprecipitated CARD9, NBP3-30275 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30275_rabbit-card9-pab-301220241556511.jpg "Immunoprecipitation:CARD9 AntibodyNBP3-30275] -Detection of human CARD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SW620 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARD9 antibody (NBP3-30275 lot 2) used for IP at 1 µg per reaction. CARD9 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-30275-1). For blotting immunoprecipitated CARD9, NBP3-30275 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CARD9 Antibody (S02-7H5) [NBP3-19592] - Western blot detection of CARD9 in K562, THP-1 cell lysates using NBP3-19592 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 62kDa.](http://images.novusbio.com/fullsize/CARD9-Antibody-S02-7H5-Western-Blot-NBP3-19592-img0001.jpg "Western Blot: CARD9 Antibody (S02-7H5) [NBP3-19592] - Western blot detection of CARD9 in K562, THP-1 cell lysates using NBP3-19592 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 62kDa.")