| Reactivity | MuSpecies Glossary |
| Applications | WB, Simple Western, IHC, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV/CA4 Glu18-Ser277 Accession # Q64444 |
| Specificity | Detects mouse Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human CA4 is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | Car4 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
Carbonic anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. The deduced amino acid sequence of mouse CA4 consists of a signal peptide (residues 1‑17), an ectodomain (residues 18‑277) and a pro peptide (residues 278‑305), which is removed in the mature form (2). The native enzyme is attached to the membrane by a GPI‑anchor. Recombinant mouse CA4 corresponds to the ectodomain and exhibits the activity as described in Activity Assay Protocol.
![Carbonic Anhydrase IV/CA4 Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Carbonic Anhydrase IV/CA4 Antibody [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
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