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Carbonic Anhydrase II/CA2 Antibody

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Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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Carbonic Anhydrase II/CA2 Antibody Summary

Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
Carbonic Anhydrase II/CA2 Antibody was produced by repeated immunizations with human erythrocytes Carbonic Anhydrase II/CA2. (Uniprot: P00918)
Localization
Cytoplasmic
Specificity
Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CA2
Purity
Multi-step
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:165000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:2000-1:10000
Application Notes
This product has been tested by ELISA, western blot, and immunohistochemistry. Expect ~29.2kDa. Researchers should determine optimal titers for applications that are not stated below.

Use in Immunohistochemistry-Frozen reported in scientific literature (PMID
Control
Carbonic Anhydrase II/CA2 Overexpression Lysate
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Reviewed Applications
Read 3 Reviews rated 5
using
NB600-919 in the following applications:

Publications
Read Publications using
NB600-919 in the following applications:

Reactivity Notes

Cross reactivity against Carbonic Anhydrase II/CA2 from other tissues and species may occur but have not been specifically determined.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Carbonic Anhydrase II/CA2 Antibody

  • CA2
  • CAII
  • CA-IIEC 4.2.1.1
  • Car2
  • Carbonate dehydratase II
  • carbonic anhydrase 2
  • carbonic anhydrase B
  • Carbonic anhydrase C
  • Carbonic Anhydrase II
  • carbonic anhydrase IICAC
  • carbonic dehydratase

Background

Carbonic Anhydrase II Antibody detects Carbonic Anhydrase II. Carbonic Anhydrase II is essential for bone resorption and osteoclast differentiation by similarity. It can hydrate cyanamide to urea and is involved in the regulation of fluid secretion into the anterior chamber of the eye. Anti-Carbonic Anhydrase II Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recent Reviews

5
5
3
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0
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2
0
1
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Amadou Sow
09/23/2014
Application: IHC
Species: Mouse

Corey .
06/24/2013
Application: WB
Species: Mouse

 
Susan Frost
10/06/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol CA2
Uniprot