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Rat Calpain 2 ELISA Kit (Colorimetric)

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ELISA: Rat Calpain 2 ELISA Kit (Colorimetric) [NBP2-74981] - Samples were spiked with high concentrations of Rat Calpain 2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Calpain 2 ELISA Kit (Colorimetric) [NBP2-74981] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL

Order Details

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Rat Calpain 2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CAPN2 in samples. No significant cross-reactivity or interference between Rat CAPN2 and analogues was observed.
Standard Curve Range
78.13 - 5000 pg/mL
Sensitivity
46.88 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.84%
Intra-Assay
CV% < 4.6%
Spike Recovery
93-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CAPN2

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Calpain 2 ELISA Kit (Colorimetric)

  • Calcium-activated neutral proteinase 2
  • calpain 2, (m/II) large subunit
  • calpain 2, large [catalytic] subunit
  • calpain 2, large subunit
  • Calpain large polypeptide L2
  • Calpain M-type
  • calpain, large polypeptide L2
  • calpain-2 catalytic subunit
  • Calpain-2 large subunit
  • CANP 2
  • CANPL2CANP2
  • CANPml
  • EC 3.4.22
  • EC 3.4.22.53
  • FLJ39928
  • M-calpain
  • mCANP
  • millimolar-calpain

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammaliancalpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist ofheterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This geneencodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites inthe 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CAPN2