![Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Formalin-fixed, paraffin-embedded human lymphoma stained with DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Immunohistochemistry-Paraffin-NBP3-08571-img0002.jpg "Immunohistochemistry-Paraffin: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Formalin-fixed, paraffin-embedded human lymphoma stained with DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, MA |
| Clone | PCRP-POLE3-2F10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07616). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human DNA Polymerase epsilon subunit 3 protein (Uniprot: Q9NRF9) |
| Localization | Nucleus, Cytoplasm |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | POLE3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 16.86 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DNA Polymerase epsilon subunit 3 Antibody (NBP3-08571)Find related products by research area.
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| Gene Symbol | POLE3 |
![Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Flow Cytometric Analysis of PFA-fixed K562 cells. DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Flow-Cytometry-NBP3-08571-img0001.jpg "Flow Cytometry: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Flow Cytometric Analysis of PFA-fixed K562 cells. DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).](http://images.novusbio.com/fullsize/DNA-Polymerase-epsilon-subunit-3-Antibody-PCRP-POLE3-2F10-Protein-Array-NBP3-08571-img0003.jpg "Protein Array: DNA Polymerase epsilon subunit 3 Antibody (PCRP-POLE3-2F10) [NBP3-08571] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DNA Polymerase epsilon subunit 3 Mouse Monoclonal Antibody (PCRP-DNA Polymerase epsilon subunit 3-2F10).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")