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BRIP1/FANCJ Antibody

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Genetic Strategies: Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human cell line A-431 shows positivity in nucleus and nuclear membrane.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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BRIP1/FANCJ Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: FNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPEL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
BRIP1/FANCJ Protein (NBP2-14361PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for BRIP1/FANCJ Antibody

  • ATP-dependent RNA helicase BRIP1
  • BACH1
  • BACH1BRCA1/BRCA2-associated helicase 1
  • BRCA1 interacting protein C-terminal helicase 1
  • BRCA1-associated C-terminal helicase 1
  • BRCA1-binding helicase-like protein BACH1
  • BRCA1-interacting protein 1
  • BRCA1-interacting protein C-terminal helicase 1
  • BRIP1
  • EC 3.6.1
  • EC 3.6.4.13
  • FACJ
  • FANCJ
  • FANCJFanconi anemia group J protein
  • FLJ90232
  • MGC126521
  • MGC126523
  • OF
  • Protein FACJ

Background

The Fanconi anemia group subunit J protein (FANCJ), also called BRIP1 or BACH1, is a helicase that interacts with the BRCT domain of BRCA1 and has a role in BRCA1-dependent DNA repair and checkpoint. Defects in FANCJ cause the autosomal recessive disorder, Fanconi anemia. BRIP1/FANCJ has a function in the Fanconi anemia pathway that is independent of BRCA1 and downstream of FANCD2 activation.

FANCG antibodies are useful for DNA repair studies and cell cycle research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol BRIP1