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BRCAA1 Antibody - BSA Free

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Western Blot: BRCAA1 Antibody [NB100-682] - Detection of BRCAA1 in MCF-7 whole cell lysate (15 ug) using 1:1,000 dilution of NB 100-682.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

BRCAA1 Antibody - BSA Free Summary

Immunogen
Reacts with residues 1046-1055 [SSKKQKRSHK] of the 136 kDa human, mouse and rat BRCAA1 protein.
Specificity
BRCAA1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARID4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 -1:1000
Application Notes
This antibody may be used for Western blot analysis where a band is seen at ~130 kDa. No other applications have been tested. Suggested Working Dilutions:* Western blot - 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MCF-7 Whole Cell Lysate

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRCAA1 Antibody - BSA Free

  • 180 kDa Sin3-associated polypeptide
  • ARID domain-containing protein 4B
  • AT rich interactive domain 4B (RBP1- like)
  • AT rich interactive domain 4B (RBP1-like)
  • AT-rich interactive domain-containing protein 4B
  • BRCAA1DKFZp313M2420
  • breast cancer-associated antigen 1
  • Breast cancer-associated antigen BRCAA1
  • breast carcinoma-associated antigen
  • Histone deacetylase complex subunit SAP180
  • Rb-binding protein homolog
  • RBBP1L1RBP1L1BCAA
  • RBP1-like protein
  • retinoblastoma binding protein 1-like 1
  • Retinoblastoma-binding protein 1-like 1
  • SAP180MGC163290
  • SIN3A-associated protein 180
  • Sin3-associated polypeptide p180

Background

The novel BRCAA1 protein's epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF-7 cells. Analysis has shown that the antigen epitope exhibited positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARID4B
Entrez