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Bovine Fibronectin Protein, CF

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Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue).
Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media ...read more
Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media ...read more
D3 mouse embryonic stem cells were expanded in KO-ES Media supplemented with Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and ...read more

Product Details

Summary
Reactivity BvSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
1030-FN-05M
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Bovine Fibronectin Protein, CF Summary

Details of Functionality
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm2.  Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives. Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is 10.0-100 ng/mL.
Source
Bovine plasma-derived Fibronectin protein
Protein/Peptide Type
Natural Proteins
Gene
FN1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Publications
Read Publications using
1030-FN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Thaw frozen material overnight at 2-8 °C. Do not disturb the product during the initial thaw event. Avoid vortexing and excessive agitation of the product.
Buffer
Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bovine Fibronectin Protein, CF

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

  1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
  2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
  3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
  4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
  5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
  6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.

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Publications for Fibronectin (1030-FN)(15)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Hamster, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Adhesion Assay, Bioassay, Cell Culture.


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Adhesion Assay
(1)
Bioassay
(13)
Cell Culture
(1)
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Human
(7)
Mouse
(6)
Rat
(1)
Hamster
(1)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 1030-FN Applications Species
Frey, N;Ouologuem, L;Blenninger, J;Siow, WX;Thorn-Seshold, J;Stöckl, J;Abrahamian, C;Fröhlich, T;Vollmar, AM;Grimm, C;Bartel, K; Endolysosomal TRPML1 channel regulates cancer cell migration by altering intracellular trafficking of E-cadherin and ?1-integrin The Journal of biological chemistry 2023-12-21 [PMID: 38141765] (Bioassay, Human, Mouse) Bioassay Human, Mouse
Zheng, C;Wei, Y;Zhang, Q;Sun, M;Wang, Y;Hou, J;Zhang, P;Lv, X;Su, D;Jiang, Y;Gumin, J;Sahni, N;Hu, B;Wang, W;Chen, X;McGrail, DJ;Zhang, C;Huang, S;Xu, H;Chen, J;Lang, FF;Hu, J;Chen, Y; Multiomics analyses reveal DARS1-AS1/YBX1-controlled posttranscriptional circuits promoting glioblastoma tumorigenesis/radioresistance Science advances 2023-08-04 [PMID: 37540752] (Bioassay, Human) Bioassay Human
M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer Oncology Letters, 2022-09-30;24(5):417. 2022-09-30 [PMID: 36245829] (Bioassay, Human) Bioassay Human
P Marchese, M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Chiesa, O Alfieri, C Foglieni Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7 International Journal of Molecular Sciences, 2021-04-14;22(8):. 2021-04-14 [PMID: 33920051] (Bioassay, Human) Bioassay Human
N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 2020-05-05;31(5):107599. 2020-05-05 [PMID: 32375049] (Cell Culture, Mouse) Cell Culture Mouse
SY Ahn, YS Maeng, YR Kim, YH Choe, HS Hwang, YM Hyun In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis Stem Cell Res Ther, 2020-02-03;11(1):44. 2020-02-03 [PMID: 32014040] (Bioassay, Human) Bioassay Human
N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin Acta Neuropathol Commun, 2018-12-12;6(1):139. 2018-12-12 [PMID: 30541620] (Bioassay, Mouse) Bioassay Mouse
SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 2018-08-13;15(1):226. 2018-08-13 [PMID: 30103769] (Bioassay, Rat) Bioassay Rat
J Yang, C Wu, I Stefanescu, A Horowitz Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies J Vis Exp, 2017-04-22;0(122):. 2017-04-22 [PMID: 28518115] (Bioassay, Mouse) Bioassay Mouse
Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells. Stem Cells, 2014-03-01;32(3):770-8. 2014-03-01 [PMID: 24155149] (Bioassay, Mouse) Bioassay Mouse
Show All 15 Publications.

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Bioinformatics

Gene Symbol FN1