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BIN1 Antibody (3B6F10) - BSA Free

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Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against HEK293 (1) and BIN1 (AA: 189-398)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of paraffin-embedded cervical cancer tissues using BIN1 mouse mAb with DAB staining.
Flow Cytometry: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis of Hela cells using BIN1 mouse mAb (green) and negative control (red).
Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mAb against human BIN1 (AA: 189-398) recombinant protein. (Expected MW is 47.1 kDa)
Western Blot: BIN1 Antibody (3B6F10) [NBP2-61714] - Analysis using BIN1 mouse mAb against C2C12 (1), A431 (2), HEK293 (3), and MCF-7 (4) cell lysate.
ELISA: BIN1 Antibody (3B6F10) [NBP2-61714] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
3B6F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-61714-0.025ml
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BIN1 Antibody (3B6F10) - BSA Free Summary

Immunogen
Purified recombinant fragment of human BIN1 (AA: 189-398) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BIN1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:100-1:2000
Theoretical MW
64.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for BIN1 Antibody (3B6F10) - BSA Free

  • AMPH2
  • Amphiphysin II
  • Amphiphysin-like protein
  • AMPHLDKFZp547F068
  • box dependant MYC interacting protein 1
  • bridging integrator 1Box-dependent myc-interacting protein 1
  • MGC10367
  • myc box-dependent-interacting protein 1
  • SH3P9

Background

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BIN1