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BIN1 Antibody (2F11)

Images

 
Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: ...read more
Immunohistochemistry of Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type (+/+) and null (-/-) mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary ...read more
Western Blot of Mouse Antibody. Lane 1: Keratinocyte derived from Bin-1 wild type. Lane 2: Keratinocyte derived from Bin-1 null mice. Load: 35 ug per lane. Primary antibody: BIN-1 antibody at 1:400 for overnight at ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
2F11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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BIN1 Antibody (2F11) Summary

Description
This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Immunogen
BIN1 Antibody (2F11) was produced in mouse by repeated immunizations with chimeric protein that encoded the human BIN1 BAR domain followed by hybridoma development. (Uniprot: O00499)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BIN1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:50000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 10-100 ul
  • Western Blot 1:500-1:1500
Application Notes
This product has been tested for use in Western Blot, ELISA, IP, and IHC. This antibody is suitable for. Specific conditions for reactivity should be optimized by the end user.
Publications
Read Publication using NBP2-21675.

Reactivity Notes

Cross-reactivity with BIN1 from other sources has not been determined

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for BIN1 Antibody (2F11)

  • AMPH2
  • Amphiphysin II
  • Amphiphysin-like protein
  • AMPHLDKFZp547F068
  • box dependant MYC interacting protein 1
  • bridging integrator 1Box-dependent myc-interacting protein 1
  • MGC10367
  • myc box-dependent-interacting protein 1
  • SH3P9

Background

BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BIN1 Antibody (NBP2-21675)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BIN1 Antibody (NBP2-21675) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BIN1
Uniprot