![Western Blot: BHMT Antibody (9E1-R) [NBP3-32082] - Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32082_mouse-bhmt-mab-9e1-r-278202415295557.jpg "Western Blot: BHMT Antibody (9E1-R) [NBP3-32082] - Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clone | 9E1-R |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein with Human BHMT aa 250-406 / 406. (Uniprot: Q93088) |
| Localization | Cytoplasm. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BHMT |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BHMT Antibody (NBP3-32082)Find related products by research area.
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| Gene Symbol | BHMT |
![Immunohistochemistry: BHMT Antibody (9E1-R) [NBP3-32082] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32082_mouse-bhmt-mab-9e1-r-298202414262613.jpg "Immunohistochemistry: BHMT Antibody (9E1-R) [NBP3-32082] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")