![Western Blot: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-beta-Tubulin I antibody Clone: SAP.4G5. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) Chicken Fibroblasts, 2.) Mouse brain extract I, 3.) Mouse brain extract II, 4.) Rat brain, 5.) Jurkat, 6.) Daudi, 7.) SK-Mel-28, 8.) G361, 9.) HeLa](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-SAP-4G5-Western-Blot-NB120-11312-img0004.jpg "Western Blot: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - Cell line lysates were separated on SDS-PAGE and probed with Monoclonal Anti-beta-Tubulin I antibody Clone: SAP.4G5. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes: 1.) Chicken Fibroblasts, 2.) Mouse brain extract I, 3.) Mouse brain extract II, 4.) Rat brain, 5.) Jurkat, 6.) Daudi, 7.) SK-Mel-28, 8.) G361, 9.) HeLa")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP, MA |
| Clone | SAP.4G5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to the C-terminal sequence of beta-tubulin isotype I (Uniprot: P07437), conjugated to BSA |
| Epitope | NB 120-11312 specifically recognizes an epitope located in the C-terminal sequence of beta tubulin isotype I of human, bovine, dog, hamster, rat, mouse, chicken and Xenopus. |
| Localization | Major constituent of microtubules. |
| Specificity | No reactivity with other tubulin isotypes is observed. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBB |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC: Use (methanolacetone and 3% paraformaldehyde-0.5% Triton-x-100 fixations). IHC: Use 10% neutral buffered formalin-alcoholic formalin-xylene, paraffin-embedded. WB: Predicted molecular weight: 55kDa. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Raw ascites |
| Preservative | 0.09% Sodium Azide |
| Purity | Unpurified |
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| Publication using NB120-11312 | Applications | Species |
|---|---|---|
| Hausrat TJ, Radwitz J, Lombino FL et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development Dev Neurobiol 2020-04-15 [PMID: 32293117] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for beta Tubulin Antibody (NB120-11312)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton(TM) X-100. Fixed cells were stained with Monoclonal Anti-beta-Tubulin I antibody Clone: SAP.4G5. The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-SAP-4G5-Immunofluorescence-NB120-11312-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (SAP.4G5) [NB120-11312] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton(TM) X-100. Fixed cells were stained with Monoclonal Anti-beta-Tubulin I antibody Clone: SAP.4G5. The antibody was developed using Goat Anti-Mouse IgG, Atto-488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
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