![Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0004.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ch, Pm, RbSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | D66 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Epitope | The epitope recognized by the antibody is localized in the C-terminal region of beta tubulin (amino acids 427-432 in sea urchin beta 2 tubulin), but is found in all isoforms of beta tubulin. |
| Localization | Cytoplasmic; Major constituent of microtubules. |
| Marker | Microtubule Marker |
| Specificity | NB600-1514 recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta Tubulin. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TUBB |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC:(3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. Predicted molecular weight: 50 kDa. |
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| Theoretical MW | ~50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) and 1.0% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta Tubulin Antibody (NB600-1514)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 0.5 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-D66-Immunocytochemistry-Immunofluorescence-NB600-1514-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 0.5 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.")
![Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - beta Tubulin antibody Total cell extract of PC-12 was separated on SDS-PAGE. The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 ug/mL antibody 2. 1 ug/mL antibody](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0001.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - beta Tubulin antibody Total cell extract of PC-12 was separated on SDS-PAGE. The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 ug/mL antibody 2. 1 ug/mL antibody")
![Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Increased expression of alpha4 subunit-containing GABARs in human epilepsy. GABAR 4 subunit protein expression in control and epilepsy cortical tissue. A representative blot from three control, three epilepsy-ganglioglioma, and three epilepsy-FCD IIa patients is shown. 30 ug of protein was used for each well and beta-tubulin expression was used as a loading control. Anti-alpha4 subunit (1:250 dilution, Novus Biologicals NB300-194, Littleton, CO) and anti-beta-tubulin (1:2000, Novus Biologicals NB600-1514) antibodies were used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/acn3.51023) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0002.jpg "Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Increased expression of alpha4 subunit-containing GABARs in human epilepsy. GABAR 4 subunit protein expression in control and epilepsy cortical tissue. A representative blot from three control, three epilepsy-ganglioglioma, and three epilepsy-FCD IIa patients is shown. 30 ug of protein was used for each well and beta-tubulin expression was used as a loading control. Anti-alpha4 subunit (1:250 dilution, Novus Biologicals NB300-194, Littleton, CO) and anti-beta-tubulin (1:2000, Novus Biologicals NB600-1514) antibodies were used. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1002/acn3.51023) licensed under a CC-BY license.")
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