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beta Tubulin Antibody (4E4)

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Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047] - Analysis of equal amounts of cell line and whole brain lysates using mouse mAb to Beta-tubulin NBP2-50047, dilution 1:5,000 in green: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS-1, [6] NIH-3T3, [7] C6 cells, [8] rat brain, and [9] mouse brain. Strong band at 50 kDa corresponds to the Beta-tubulin proteins.
Western Blot: beta Tubulin Antibody (4E4) [NBP2-50047] - Analysis of equal amounts of cell line and whole brain lysates using mouse mAb to Beta-tubulin NBP2-50047, dilution 1:5,000 in green: [1] protein standard (red), ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (4E4) [NBP2-50047] - HeLa cells stained with NBP2-50047 (green) and chicken antibody to Lamin A+C (NBP2-25152, red) and DNA in blue. NBP2-50047 reveals ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-50047AF350
Alexa Fluor 405
NBP2-50047AF405
Alexa Fluor 488
NBP2-50047AF488
Alexa Fluor 532
NBP2-50047AF532
Alexa Fluor 594
NBP2-50047AF594
Alexa Fluor 647
NBP2-50047AF647
Alexa Fluor 700
NBP2-50047AF700
Alexa Fluor 750
NBP2-50047AF750
Biotin
NBP2-50047B
CoraFluor 1
NBP2-50047CL1
DyLight 350
NBP2-50047UV
DyLight 405
NBP2-50047V
DyLight 488
NBP2-50047G
DyLight 550
NBP2-50047R
DyLight 594
NBP2-50047DL594
DyLight 650
NBP2-50047C
DyLight 680
NBP2-50047FR
DyLight 755
NBP2-50047IR
FITC
NBP2-50047F
HRP
NBP2-50047H
Janelia Fluor 525
NBP2-50047JF525
Janelia Fluor 549
NBP2-50047JF549
Janelia Fluor 646
NBP2-50047JF646
Janelia Fluor 669
NBP2-50047JF669
mFluor Violet 450 SE
NBP2-50047MFV450
mFluor Violet 500 SE
NBP2-50047MFV500
mFluor Violet 610 SE
NBP2-50047MFV610
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beta Tubulin Antibody (4E4) Summary

Immunogen
Pig brain beta Tubulin. [UniProt# Q767L7]
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TUBB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Western Blot 1:5000 - 1:10000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-50047 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for beta Tubulin Antibody (4E4)

  • beta polypeptide
  • beta Tubulin
  • MGC117247
  • OK/SW-cl.56
  • TUBB
  • TUBB5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-50047
beta Tubulin Antibody (4E4)
Species: Hu, Mu, Rt, Pm
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Publications for beta Tubulin Antibody (NBP2-50047)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol TUBB
Uniprot