![Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Western-Blot-NBP2-67406-img0007.jpg "Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SI79-01 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human beta-NGF aa 192-241 / 241. (SwissProt: P01138 Human; SwissProt: P01139 Mouse; SwissProt: P25427 Rat) |
| Localization | Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NGF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for beta-NGF Antibody (NBP2-67406)Find related products by research area.
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| Gene Symbol | NGF |
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta-NGF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0008.jpg "Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta-NGF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0001.jpg "Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunocytochemistry-Immunofluorescence-NBP2-67406-img0002.jpg "Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0004.jpg "Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0005.jpg "Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-NGF-Antibody-SI79-01-Immunohistochemistry-Paraffin-NBP2-67406-img0006.jpg "Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")