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beta-NGF Antibody (5F10)

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Immunohistochemistry: beta-NGF Antibody (5F10) [NBP3-26112] - Image of beta-NGF Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: beta-NGF Antibody (5F10) [NBP3-26112] - Image of beta-NGF Antibody (5F10) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen ...read more
Western Blot: beta-NGF Antibody (5F10) [NBP3-26112] - Positive Western Blot detected in: U-87 whole cell lysate, Rat Brain whole cell lysate. All lanes: beta-NGF Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
5F10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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beta-NGF Antibody (5F10) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human beta-NGF [UniProt P01138]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NGF
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for beta-NGF Antibody (5F10)

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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beta-NGF Antibody (5F10)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NGF