![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0019.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Whole cell extract of human fibroblasts was separated on SDS-PAGE and blotted with Monoclonal Anti-beta-Actin. The antibody was developed with Goat Anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lanes A: Antibody dilution 1:5,000 B: Negative control (only secondary antibody).")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Tr, Ze, Dr(-)Species Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, MA, KD, KO |
| Clone | AC-15 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | This is the purified version of NB600-501. |
| Immunogen | This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
| Epitope | N-terminus of the beta isoform of actin. |
| Specificity | Beta-actin (AC-15). In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTB |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:25-1:100; matrix was 12-230 kDa. |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in HS-68 cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Immunocytochemistry-Immunofluorescence-NB110-67828-img0006.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in HS-68 cells.")
![Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in breast, Epithelium 40X.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Immunohistochemistry-NB110-67828-img0007.jpg "Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Staining in breast, Epithelium 40X.")
![Flow Cytometry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Flow-Cytometry-NB110-67828-img0002.jpg "Flow Cytometry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of HeLa cells using mouse Monoclonal beta-Actin antibody (orange) and Isotype control antibody (blue).")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Chicken fibroblast cells probed with monoclonal PA to Beta Actin, clone AC-15 (0.5 ug/ml). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0001.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Chicken fibroblast cells probed with monoclonal PA to Beta Actin, clone AC-15 (0.5 ug/ml). The antibody was developed with Alkaline Phosphatase conjugate and a NBT/BCIP substrate.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0010.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis of beta-Actin in bovine whole skeletal muscle lysate. Image from verified customer review.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0011.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in CHO, GC-1, HeLa, Cos-7 and SH-SY5Y cell lysates using anti-beta-Actin antibody. The primary antibody was used at a dilution of 1:10,000 for 1 hour. Image from verified customer review.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0012.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0013.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - beta-Actin expression in human glioma cells using anti-beta-Actin antibody. Image from verified customer review.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - GFAP and IL-6 are elevated in cultures of spinal cord compared to cortical astrocytes. ((G, H) Analysis of protein homogenates from brain or spinal cord astrocyte cultures also demonstrated higher levels of GFAP expression, but no differences in ALDH1L1. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0180697), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0014.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - GFAP and IL-6 are elevated in cultures of spinal cord compared to cortical astrocytes. ((G, H) Analysis of protein homogenates from brain or spinal cord astrocyte cultures also demonstrated higher levels of GFAP expression, but no differences in ALDH1L1. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0180697), licensed under a CC-BY license.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0015.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HEK293T cells transfected with Niaph virus Matrix Protein, expression 24h later. This image was submitted via customer review.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HSP90 and HSP70 interact with PABPN1. Interaction of PABPN1 with HSP90. Lysates from HEK293 cells transfected with Flag-HSP90 and GFP-tagged A17-PABPN1 were immunoprecipitated with an anti-Flag antibody. Immunoprecipitates (IP) were analyzed by Western blot (IB).Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0138936), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0016.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - HSP90 and HSP70 interact with PABPN1. Interaction of PABPN1 with HSP90. Lysates from HEK293 cells transfected with Flag-HSP90 and GFP-tagged A17-PABPN1 were immunoprecipitated with an anti-Flag antibody. Immunoprecipitates (IP) were analyzed by Western blot (IB).Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0138936), licensed under a CC-BY license.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0017.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta-actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.")
![Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration. Western blot analysis showing the expression of MMP-11 protein in five oral cancer cell lines. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113129), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Western-Blot-NB110-67828-img0018.jpg "Western Blot: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - MMP-11 expression in oral cancer cell lines and the MMP-11 knockdown in SCC9 cells reduce cell migration. Western blot analysis showing the expression of MMP-11 protein in five oral cancer cell lines. Beta-Actin is included as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0113129), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Immunocytochemistry-Immunofluorescence-NB110-67828-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Beta-actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NB600-501) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.")
![Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Immunocytochemistry-Immunofluorescence-NB110-67828-img0004.jpg "Immunocytochemistry/Immunofluorescence: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-Beta-Actin, Clone No. AC-15 (NB600-501).")
![Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Immunohistochemistry-NB110-67828-img0005.jpg "Immunohistochemistry: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Double exposure micrographs of longitudinal, expanded (a,b) and transverse (c) semi-thin cryosections of chicken gizzard muscle double labeled with Monoclonal Anti-alpha-Actin and polyclonal antibodies to chicken gizzard myosin (red). d-f. Longitudinal, expanded cryosections of chicken gizzard muscle double labeled with polyclonal Anti-alpha-Actinin (green, d, e, red, f) in combination with antibodies to chicken gizzard myosin, antibodies to chicken desmin and Monoclonal Anti-Beta Actin,(f, green). Bars, 10 mm. From Dr. J. V. Small, Institute of Molecular Biology, Academy of Sciences, Salzburg.")
![Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y,](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Simple-Western-NB110-67828-img0008.jpg "Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Electropherogram image(s) of corresponding Simple Western lane view. Beta-Actin antibody was used at 1:500 dilution on Hela, MCF-7, SH-SY5Y,")
![Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y,](http://images.novusbio.com/fullsize/beta-Actin-Antibody-AC-15-Purified-Simple-Western-NB110-67828-img0009.jpg "Simple Western: beta-Actin Antibody (AC-15) - Purified [NB110-67828] - Analysis using the HRP conjugate of NB600-501. Image shows a specific band for Beta-Actin in 0.2 mg/mL of Hela, MCF-7, SH-SY5Y,")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")