![Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Western-Blot-NB500-317-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.")
| Reactivity | Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)Species Glossary |
| Applications | WB, ELISA, Flow, Func, ICC/IF, IHC, IP |
| Clone | B2M-01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-44521 | |||
| FITC | NB110-60015 | |||
| HRP | NBP1-44520 | |||
| Immunogen | Purified human beta 2 Microglobulin (NM_004048.2). |
| Specificity | The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | B2M |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. IHC-P: positive tissue: kidney glomeruli. Flow cytometry: positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. IP: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells. |
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| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
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| Publications using NB500-317 | Applications | Species |
|---|---|---|
| Rivino L, Tan AT, Chia A et al. Defining CD8+ T Cell Determinants during Human Viral Infection in Populations of Asian Ethnicity J Immunol 2013-10-15 [PMID: 24058176] (ELISA, Human) Details: This citation used the HRP version of this antibody. |
ELISA | Human |
| Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01 | ||
| Tabandeh MR, Jozaie S, Ghotbedin Z, Gorani S Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfk beta, NLRP3, JNK signaling pathways Research in veterinary science 2022-08-01 [PMID: 35933803] (WB, Bovine) | WB | Bovine |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
IHC-P | Human | 03/25/2020 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta 2-Microglobulin Antibody (NB500-317)Find related products by research area.
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| Gene Symbol | B2M |
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Immunocytochemistry-Immunofluorescence-NB500-317-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0012.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0008.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0010.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-Flow-Cytometry-NB500-317-img0011.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.")
![ELISA: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-01-ELISA-NB500-317-img0013.jpg "ELISA: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody. Image using the HRP format of this antibody.")
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