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beta 2-Microglobulin Antibody (B2M-01) [FITC]

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Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [FITC] [NB110-60015] - Staining of beta2-microglobulin in human peripheral blood with anti-beta 2-microglobulin (B2M-01) FITC.
Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [FITC] [NB110-60015] - Staining of beta2-microglobulin in human peripheral blood with anti-beta 2-microglobulin (B2M-01) FITC.

Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)Species Glossary
Applications Flow, ICC/IF
Clone
B2M-01
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
NBP1-44521
HRP
NBP1-44520
Unconjugated
NB500-317
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-317
BSA Free
NB500-317
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beta 2-Microglobulin Antibody (B2M-01) [FITC] Summary

Immunogen
Purified human beta 2 Microglobulin (NM_004048.2).
Specificity
The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/ Immunofluorescence
Application Notes
Purity is > 95% (by SDS-PAGE)
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
beta 2-Microglobulin Overexpression Lysate

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Size Exclusion Chromatography

Alternate Names for beta 2-Microglobulin Antibody (B2M-01) [FITC]

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for beta 2-Microglobulin Antibody (NB110-60015) (0)

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Control Lysate(s)

beta 2-Microglobulin Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Product PDFs

  SDS
  CofA Lot M58J
  PDF Datasheet

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Bioinformatics

Gene Symbol B2M