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beta 2-Microglobulin Antibody (B2M-01) [HRP]

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Western Blot: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human beta2-microglobulin (B2M-01). Lane 1: RAJI human ...read more
ELISA: beta 2-Microglobulin Antibody (B2M-01) [HRP] [NBP1-44520] - Human serum using anti-HLA-G (capture) and anti-beta2 microglobulin HRP (detection).

Product Details

Summary
Reactivity Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)Species Glossary
Applications WB, ELISA
Clone
B2M-01
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Concentration
0.2 mg/ml

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beta 2-Microglobulin Antibody (B2M-01) [HRP] Summary

Description
Store in the dark. Avoid prolonged exposure to light.
Immunogen
Purified human beta 2 Microglobulin (NM_004048.2).
Specificity
The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Western Blot 1 ug/ml
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Publications
Read Publication using
NBP1-44520 in the following applications:

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Stabilizing phosphate buffered saline (PBS)
Preservative
0.01% Thimerosal
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for beta 2-Microglobulin Antibody (B2M-01) [HRP]

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for beta 2-Microglobulin Antibody (NBP1-44520)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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Bioinformatics

Gene Symbol B2M