![Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Western-Blot-NBP2-61698-img0004.jpg "Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against human BAG1 (AA: 219-346) recombinant protein. (Expected MW is 40.6 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF |
| Clone | 1E4B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Bag-1 (AA: 219-346) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BAG1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 38.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Bag-1 Antibody (NBP2-61698)Find related products by research area.
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BAG3 - Hsp70 is my friend! The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1.... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BAG1 |
![Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Immunocytochemistry-Immunofluorescence-NBP2-61698-img0003.jpg "Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.")
![Flow Cytometry: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Flow-Cytometry-NBP2-61698-img0002.jpg "Flow Cytometry: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis of Hela cells using BAG1 mouse mAb (green) and negative control (red).")
![Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-Western-Blot-NBP2-61698-img0005.jpg "Western Blot: Bag-1 Antibody (1E4B5) [NBP2-61698] - Analysis using BAG1 mAb against HEK293 (1) and BAG1 (AA: 219-346)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: Bag-1 Antibody (1E4B5) [NBP2-61698] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Bag-1-Antibody-1E4B5-ELISA-NBP2-61698-img0001.jpg "ELISA: Bag-1 Antibody (1E4B5) [NBP2-61698] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)")
![Western Blot Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Western_Blot_5287.jpg)
![Simple Western Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Simple_Western_16990.jpg)
![Knockout Validated Catalase Antibody [Unconjugated]](https://images.novusbio.com/images2/Catalase_AF3398_Knockout_Validated_24032.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")