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Axin-2 Antibody - BSA Free

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Western Blot: Axin-2 Antibody [NBP1-76526] - Mouse lung lysate at 1 ug/mL.
Western Blot: Axin-2 Antibody [NBP1-76526] - Mouse lung lysate at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: Axin-2 Antibody [NBP1-76526] - HepG2 cells with AXIN2Antibody at 20 ug/ml.
Immunohistochemistry: Axin-2 Antibody [NBP1-76526] - Mouse lung tissue antibody at 5 ug/mL.
Immunocytochemistry/ Immunofluorescence: Axin-2 Antibody [NBP1-76526] - HepG2 cells with Antibody at 20 ug/ml.
Immunohistochemistry: Axin-2 Antibody [NBP1-76526] - Rat lung tissue at 5 ug/mL.
Immunohistochemistry-Paraffin: Axin-2 Antibody [NBP1-76526] - Staining of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-76526AF350
Alexa Fluor 405
NBP1-76526AF405
Alexa Fluor 488
NBP1-76526AF488
Alexa Fluor 532
NBP1-76526AF532
Alexa Fluor 594
NBP1-76526AF594
Alexa Fluor 647
NBP1-76526AF647
Alexa Fluor 700
NBP1-76526AF700
Alexa Fluor 750
NBP1-76526AF750
Biotin
NBP1-76526B
CoraFluor 1
NBP1-76526CL1
DyLight 350
NBP1-76526UV
DyLight 405
NBP1-76526V
DyLight 488
NBP1-76526G
DyLight 550
NBP1-76526R
DyLight 594
NBP1-76526DL594
DyLight 650
NBP1-76526C
DyLight 680
NBP1-76526FR
DyLight 755
NBP1-76526IR
FITC
NBP1-76526F
HRP
NBP1-76526H
Janelia Fluor 525
NBP1-76526JF525
Janelia Fluor 549
NBP1-76526JF549
Janelia Fluor 585
NBP1-76526JF585
Janelia Fluor 635
NBP1-76526JF635
Janelia Fluor 646
NBP1-76526JF646
Janelia Fluor 669
NBP1-76526JF669
PerCP
NBP1-76526PCP
mFluor Violet 450 SE
NBP1-76526MFV450
mFluor Violet 500 SE
NBP1-76526MFV500
mFluor Violet 610 SE
NBP1-76526MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-76526-0.025mg
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Axin-2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. The immunogen is located within amino acids 780 - 830 of AXIN2. Amino Acid Squence: KEQLSKKGNYRYYFKKASDE
Specificity
Axin-2 antibody is not predicted to cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AXIN2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
Theoretical MW
85, 93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Axin-2 Peptide (NBP1-76526PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Axin-2 Antibody - BSA Free

  • AXIL
  • axin 2
  • Axin2
  • Axin-2
  • Axin-like protein
  • Axis inhibition protein 2
  • Conductin
  • DKFZp781B0869
  • MGC10366
  • MGC126582

Background

Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AXIN2
Uniprot