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ATP7b Antibody

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Immunocytochemistry/Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.
Immunocytochemistry/ Immunofluorescence: ATP7b Antibody [NBP2-58923] - Staining of human cell line CACO-2 shows localization to the Golgi apparatus.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ATP7b Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: ATVLYNPSVISPEELRAAIEDMGFEASVVSESCSTNPLGNHSAGNSMVQTTDGTPTSVQEVAPHTGRLPANHAPDILAKSPQSTRAVAPQKCFLQIKGMTCASCVSNIERNLQKEAGVLSVLV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP7B
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATP7b Recombinant Protein Antigen (NBP2-58923PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATP7b Antibody

  • ATPase, Cu(2+)- transporting, beta polypeptide
  • ATPase, Cu++ transporting, beta polypeptide
  • Copper pump 2
  • copper-transporting ATPase 2
  • EC 3.6.3
  • EC 3.6.3.4
  • PWD
  • WC1
  • WD
  • Wilson disease-associated protein
  • WNDATPase, Cu++ transporting, beta polypeptide (Wilson disease)

Background

ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; form A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ATP7B