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ATG4C Antibody

Images

 
Western Blot: ATG4C Antibody [NBP1-89043] - Analysis in control (vector only transfected HEK293T lysate) and ATG4C over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ATG4C Antibody [NBP1-89043] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: ATG4C Antibody [NBP1-89043] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ATG4C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFV
Predicted Species
Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG4C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ATG4C Protein (NBP1-89043PEP)
Publications
Read Publication using NBP1-89043.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ATG4C Antibody

  • APG4 autophagy 4 homolog C (S. cerevisiae)
  • APG4 autophagy 4 homolog C
  • APG4C
  • ATG4 autophagy related 4 homolog C (S. cerevisiae)
  • AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae)
  • AUTL1
  • AUTL3APG4-C
  • AUT-like 1, cysteine endopeptidase
  • AUT-like 3 cysteine endopeptidase
  • autophagin-3
  • Autophagy-related cysteine endopeptidase 3
  • Autophagy-related protein 4 homolog C
  • cysteine protease ATG4C
  • EC 3.4.22
  • EC 3.4.22.-
  • FLJ14867

Background

ATG4C is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATG4C Antibody (NBP1-89043)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATG4C Antibody (NBP1-89043) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for ATG4C Antibody (NBP1-89043)

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Blogs on ATG4C.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

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Bioinformatics

Gene Symbol ATG4C