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ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

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Protein Array: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ATF2 Antibody (PCRP-ATF2-1B4).
Protein Array: ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free [NBP3-14001] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ATF2 Antibody (PCRP-ATF2-1B4).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IP, MA
Clone
PCRP-ATF2-1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14001AF350
Alexa Fluor 405
NBP3-14001AF405
Alexa Fluor 488
NBP3-14001AF488
Alexa Fluor 532
NBP3-14001AF532
Alexa Fluor 594
NBP3-14001AF594
Alexa Fluor 647
NBP3-14001AF647
Alexa Fluor 700
NBP3-14001AF700
Alexa Fluor 750
NBP3-14001AF750
Allophycocyanin
NBP3-14001APC
Biotin
NBP3-14001B
CoraFluor 1
NBP3-14001CL1
DyLight 350
NBP3-14001UV
DyLight 405
NBP3-14001V
DyLight 488
NBP3-14001G
DyLight 550
NBP3-14001R
DyLight 594
NBP3-14001DL594
DyLight 650
NBP3-14001C
DyLight 680
NBP3-14001FR
DyLight 755
NBP3-14001IR
FITC
NBP3-14001F
HRP
NBP3-14001H
Janelia Fluor 525
NBP3-14001JF525
Janelia Fluor 549
NBP3-14001JF549
Janelia Fluor 585
NBP3-14001JF585
Janelia Fluor 635
NBP3-14001JF635
Janelia Fluor 646
NBP3-14001JF646
Janelia Fluor 669
NBP3-14001JF669
PE
NBP3-14001PE
PerCP
NBP3-14001PCP
Unconjugated
NBP3-13855-100ug
mFluor Violet 450 SE
NBP3-14001MFV450
mFluor Violet 500 SE
NBP3-14001MFV500
mFluor Violet 610 SE
NBP3-14001MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-13855-100ug
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ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13855).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human ATF2 protein (Uniprot: P15336)
Localization
Nucleus
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ATF2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Protein Array
  • Western Blot 1-2 ug/ml
Theoretical MW
54.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in mouse and rat.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ATF2 Antibody
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NBP3-14001
ATF2 Antibody (PCRP-ATF2-1B4) - Azide and BSA Free
Species: Hu
Applications: WB, Flow, ICC/IF, IP, MA

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol ATF2