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ARNT/HIF-1 beta Antibody (775146)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ARNT/HIF-1 beta Monoclonal Antibody (Catalog # MAB5630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ARNT/HIF-1 beta at approximately 90-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 ...read more
ARNT/HIF-1 beta was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ARNT/HIF-1 beta Monoclonal Antibody (Catalog # MAB5630) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
775146
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5630-SP
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ARNT/HIF-1 beta Antibody (775146) Summary

Immunogen
E. coli-derived recombinant human ARNT/HIF‑1 beta
Asp517-Ala691
Accession # P27540
Specificity
Detects human ARNT/HIF‑1 beta in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ARNT
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARNT/HIF-1 beta Antibody (775146)

  • ARNT protein
  • ARNT
  • aryl hydrocarbon receptor nuclear translocator
  • BHLHE2
  • Class E Basic Helix-Loop-Helix Protein 2
  • Dioxin Receptor, Nuclear Translocator
  • HIF1 beta
  • HIF-1 beta
  • HIF1B
  • HIF1BETA
  • HIF-1beta
  • HIF-1-beta
  • HIF1-beta
  • hypoxia-inducible factor 1, beta subunit
  • Hypoxia-inducible factor 1-beta
  • TANGO

Background

ARNT (Aryl hydrocarbon nuclear translocator; also HIF-1 beta , Hypoxia-inducible factor 1-beta) is an 87-97 kDa member of the bHLH-PAS subclass of the bHLH family of transcription factors. It is widely expressed, and serves as part of both the NF kappa B and aryl hydrocarbon receptor (AHR) signaling nodes. For the AHR, the AHR is normally inactive and bound to hsp90 in the cytosol. Following ligand binding, the AHR complex enters the nucleus, dissociates from hsp90, dimerizes with ARNT, and then binds response elements of select genes. Human ARNT is 789 amino acids (aa) in length. It contains a DNA-binding region (aa 87‑143), two PAS domains (aa 161‑235 and 362‑458) and one PAC region (aa 424‑467). There are multiple potential splice forms. Two alternate start sites are found at Met10 and Met354, there is a deletion of aa 329‑755 and 77‑91, and an Ala substitution for aa 328‑789.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ARNT/HIF-1 beta
Antibody (775146)
MAB5630
ARNT/HIF-1 beta Antibody (775146)
Species: Hu
Applications: WB, ICC/IF

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for ARNT/HIF-1 beta Antibody (MAB5630). (Showing 1 - 3 of 3 FAQ).

  1. Is this target appropriate for use in Chromatin Research?
    • Yes; here is a list of research areas that we have deemed appropriate for the target "ARNT/HIF-1 beta": Angiogenesis, Autophagy, Cancer, Cellular Markers, Chromatin Research, HIF Target Genes, Hypoxia, Transcription Factors and Regulators, Cardiovascular Biology, Lipid and Metabolism.
  2. For use in Western Blot with HIF-1 beta antibodies, what molecular weight of the band should I expect to see?
    • The theoretical molecular weight determined by our technical team for ARNT/HIF-1 beta antibodies is 86.6 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product, allowing the tested application/species to fall under our 100% guarantee. Please check out our Innovator's Reward Program if you decide to test a primary antibody with a species or application that is not currently listed. Please note that the Innovator's Reward Program only applies to our primary antibodies.

Secondary Antibodies

 

Isotype Controls

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Blogs on ARNT/HIF-1 beta.

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HIF-2 alpha: HIF1A's Homologue with Similar and Divergent Functions
HIF-2 alpha is a member of the heterodimeric hypoxia-inducible factors/HIFs family (HIF-1, HIF-2, and HIF-3) which contains a common beta subunit but differ in their alpha subunits. Also called as EPAS1 or Mop2, HIF-2 alpha regulates cellular adapt...  Read full blog post.
HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.
HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.
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Bioinformatics

Gene Symbol ARNT
Uniprot