![Western Blot: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody NB100-57484 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ARID1B-Antibody-Western-Blot-NB100-57484-img0007.jpg "Western Blot: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody NB100-57484 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1300 and 1350 of human AT rich interactive domain 1B using the numbering given in entry NP_787059.2 (GeneID 57492). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ARID1B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57484] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (NB100-57484). Detection: DAB](http://images.novusbio.com/fullsize/ARID1B-Antibody-Immunohistochemistry-NB100-57484-img0008.jpg "Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57484] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (NB100-57484). Detection: DAB")
![Western Blot: ARID1B Antibody [NB100-57484] - Detection of Human ARID1B on HeLa whole cell lysates using NB100-57484. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-47485.](http://images.novusbio.com/fullsize/ARID1B-Antibody-Western-Blot-NB100-57484-img0002.jpg "Western Blot: ARID1B Antibody [NB100-57484] - Detection of Human ARID1B on HeLa whole cell lysates using NB100-57484. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-47485.")
![Immunoprecipitation: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57484 (lot NB100-57484-3) used for IP at 3 ug per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-57484-2) and rabbit anti-ARID1B antibody NB100-57485. For blotting immunoprecipitated ARID1B, NB100-57484 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ARID1B-Antibody-Immunoprecipitation-NB100-57484-img0009.jpg "Immunoprecipitation: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57484 (lot NB100-57484-3) used for IP at 3 ug per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-57484-2) and rabbit anti-ARID1B antibody NB100-57485. For blotting immunoprecipitated ARID1B, NB100-57484 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")