![Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Western-Blot-NBP2-20415-img0031.jpg "Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, ChIP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SMARCC1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ChIP assay Assay-dependent dilution |
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| Theoretical MW | 123 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-20415 | Applications | Species |
|---|---|---|
| Sauls k, Greco TM, Wang L et al. Initiating Events in Direct Cardiomyocyte Reprogramming. Cell Rep. 2018-02-13 [PMID: 29444441] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for SMARCC1 Antibody (NBP2-20415)Find related products by research area.
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![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0008.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.")
![Chromatin Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 ug of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-20415-img0027.jpg "Chromatin Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 ug of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.")
![Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Western-Blot-NBP2-20415-img0029.jpg "Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMARCC1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20415-img0005.jpg "Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMARCC1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0002.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0003.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0004.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse duodenum dilution: 1:50](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0006.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse duodenum dilution: 1:50")
![Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse prostate dilution: 1:500.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunohistochemistry-Paraffin-NBP2-20415-img0007.jpg "Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded mouse prostate dilution: 1:500.")
![Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 ug of SMARCC1 antibody 5% SDS-PAGE The mmunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/SMARCC1-Antibody-Immunoprecipitation-NBP2-20415-img0010.jpg "Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 ug of SMARCC1 antibody 5% SDS-PAGE The mmunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.")
![Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (NBP2-20415) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20415_rabbit-polyclonal-smarcc1-antibody-91020242001053.jpg "Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (NBP2-20415) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCC1 stained by SMARCC1 antibody [C2C3], C-term (NBP2-20415) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-20415_rabbit-polyclonal-smarcc1-antibody-910202420134091.jpg "Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SMARCC1 stained by SMARCC1 antibody [C2C3], C-term (NBP2-20415) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.")
![Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20415_rabbit-polyclonal-smarcc1-antibody-1610202419414520.jpg "Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Western_Blot_5829.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11663.jpg)
![Immunocytochemistry Brg1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Brg1_AF5738_Immunocytochemistry_11664.jpg)
![COCO/DAND5 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "COCO/DAND5 [Unconjugated]")
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/DATA_PSG1_DPSG10_ELISA_797.jpg)
![N/A PSG1 [HRP]](https://images.novusbio.com/images2/PSG-1_DPSG10_ELISA_164.jpg)
![Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Chromatin_Immunoprecipitation_11218.jpg)
![Immunocytochemistry Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct-3_4_AF1759_Immunocytochemistry_7093.jpg)
![Simple Western Oct-3/4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Oct3_4_AF1759_Simple_Western_20183.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")