Arginase 1/ARG1/liver Arginase Antibody

Arginase 1 (ARG1), also known as liver arginase, is a metalloenzyme that is a member of the ureohydrolase superfamily and arginase family (1). ARG1 is known for its role in the urea cycle in catalyzing the conversion of L-arginine into urea and L-ornithine (1). Arginase has two distinct isoforms, with ARG1 being expressed primarily in the liver and ARG2 in extrahepatic tissues (1). Human ARG1 is synthesized as 322 amino acids (aa) in length with a theoretical molecular weight of 35 kDa (1,2). Three ARG1 monomers can form a highly active homotrimer of 105 kDa (1). A key structural feature of the arginase protein is the binuclear magnesium (Mn2+) ions at its core (1).

Arginase and nitric oxidase synthase (NOS) compete for the same L-arginine substrate, creating a delicate balance between pathways (1). Furthermore, bioavailability of L-arginine and ARG1 expression has been implicated in several pathologies including vascular disease, neuronal disease, cardiovascular disease, immune dysfunction, inflammation, and cancer (1,3-5). For instance, ARG1 functions as a macrophage marker, defining the M2 population, while inducible nitric oxide synthase (iNOS) characterizes the M1 population; impaired M1/M2 polarization and changes in ARG1 expression is observed in diseases such as arteriogenesis, asthma, pulmonary fibrosis, and inflammatory bowel disease (1,3). In humans, arginase deficiency, known as argininemia, is an autosomal recessive metabolic disorder characterized by elevated ammonia (hyperammonemia) levels and arginine accumulation (6). Given that many arginase-associated diseases are characterized by upregulation in expression of ARG1, ARG2, or both, arginase inhibitors are currently being studied as a potential therapeutic approach (1,4).

References

1. S Clemente, G., van Waarde, A., F Antunes, I., Domling, A., & H Elsinga, P. (2020). Arginase as a Potential Biomarker of Disease Progression: A Molecular Imaging Perspective. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms21155291

2. Uniprot (P05089)

3. Kieler, M., Hofmann, M., & Schabbauer, G. (2021). More than just protein building blocks: How amino acids and related metabolic pathways fuel macrophage polarization. The FEBS Journal. Advance online publication. https://doi.org/10.1111/febs.15715

4. Shosha, E., Fouda, A. Y., Narayanan, S. P., Caldwell, R. W., & Caldwell, R. B. (2020). Is the Arginase Pathway a Novel Therapeutic Avenue for Diabetic Retinopathy?. Journal of Clinical Medicine. https://doi.org/10.3390/jcm9020425

5. Correale J. (2021). Immunosuppressive Amino-Acid Catabolizing Enzymes in Multiple Sclerosis. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.600428

6. Morales, J. A., & Sticco, K. L. (2020). Arginase Deficiency. In StatPearls. StatPearls Publishing.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, WB, Western Blot.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NB100-59740	Applications	Species
Zhou J, Zhang C, Yang X et al. Study on the effect of sevoflurane on the cognitive function of aged rats based on the activation of cortical microglia Ibrain 2021-12-01 (WB, Rat)	WB	Rat
Kim H, You M, Sung S et al. Possible involvement of microglial P2RY12 and peripheral IL-10 in postpartum depression Frontiers in Cellular Neuroscience 2023-06-15 [PMID: 37396924] (Western Blot)	Western Blot
Liu S, Hur YH, Cai X et al. A tissue injury sensing and repair pathway distinct from host pathogen defense Cell 2023-04-24 [PMID: 37098344] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Lopez R, Li B, Keren-Shaul H et al. DestVI identifies continuums of cell types in spatial transcriptomics data Nature Biotechnology 2022-09-01 [PMID: 35449415] (Immunohistochemistry-Frozen)	Immunohistochemistry-Frozen
Li Y, Acosta F, Quan Y et al. Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model STAR Protocols 2022-03-01 [PMID: 35005642] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ghallab A, Myllys M, Friebel A et al. Spatio-Temporal Multiscale Analysis of Western Diet-Fed Mice Reveals a Translationally Relevant Sequence of Events during NAFLD Progression Cells 2021-09-23 [PMID: 34685496] (IF/IHC, Mouse)	IF/IHC	Mouse
Shao D, Li K, You M et al. Macrophage polarization by plasma sprayed ceria coatings on titanium-based implants: Cerium valence state matters iScience 2021-06-18 [PMID: 34142044] (Mouse)		Mouse
Sadler R, Cramer JV, Heindl S et al. Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms J. Neurosci. 2020-01-29 [PMID: 31889008] (IF/IHC, Mouse)	IF/IHC	Mouse
Rodet F, Capuz A, Ozcan BA et al. PC1/3 KD Macrophages Exhibit Resistance to the Inhibitory Effect of IL-10 and a Higher TLR4 Activation Rate, Leading to an Anti-Tumoral Phenotype Cells 2019-11-22 [PMID: 31766635] (WB, Rat)	WB	Rat
McLane V D, Kumar S et al. Morphine-potentiated cognitive deficits correlate to suppressed hippocampal iNOS RNA expression and an absent type 1 interferon response in LP-BM5 murine AIDS. J Neuroimmunol 2018-06-15 [PMID: 29526406] (IF/IHC, Mouse)	IF/IHC	Mouse
C Carmona-Fo, M Deforet, L Akkari, CB Thompson, JA Joyce, JB Xavier Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 2017-02-28;0(0):. 2017-02-28 [PMID: 28246332] (Immunocytochemistry/ Immunofluorescence, Human)	Immunocytochemistry/ Immunofluorescence	Human
Jang E, Kim JH, Lee S et al. Phenotypic polarization of activated astrocytes: the critical role of lipocalin-2 in the classical inflammatory activation of astrocytes. J Immunol. 2013-11-15 [PMID: 24089194] (ICC/IF, FLOW, IHC-Fr, Mouse)	ICC/IF, FLOW, IHC-Fr	Mouse
Jang E, Lee S, Kim JH et al. Secreted protein lipocalin-2 promotes microglial M1 polarization FASEB J 2012-12-04 [PMID: 23207546] (FLOW, ICC/IF, IF/IHC, Mouse)	FLOW, ICC/IF, IF/IHC	Mouse
Yu M, Eckart MR, Morgan AA et al. Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma. J Clin Invest. 2011-07-01 [PMID: 21737883]
Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O. All-trans retinoic acid modifies the expression of clock and disease marker genes. J Nutr Biochem. 2011-04-14 [PMID: 21497500]
Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O. Caffeine alters circadian rhythms and expression of disease and metabolic markers. Int J Biochem Cell Biol;43(5):829-38. 2011-05-01 [PMID: 21352949]
Jiang M, Ding Y, Su Y et al. Arginase-flotillin interaction brings arginase to red blood cell membrane. FEBS Lett 2006-12-11 [PMID: 17113085]
Show All 17 Publications. Collapse Publications.

Array NB100-59740

• Arginase 1/ARG1/liver Arginase Antibodies
• Arginase 1/ARG1/liver Arginase ELISA Kit
• Arginase 1/ARG1/liver Arginase Kit
• Arginase 1/ARG1/liver Arginase Lysate
• Arginase 1/ARG1/liver Arginase Proteins
• Arginase 1/ARG1/liver Arginase Proteins and Enzyme