![Western Blot: Apc4 Antibody [NB100-59824] - Detection of Human and Mouse APC4 on HeLa whole cell lysates using NB100-59824. APC4 was also immunoprecipitated by rabbit anti-APC4 antibody NB100-59823.](http://images.novusbio.com/fullsize/Apc4-Antibody-Western-Blot-NB100-59824-img0002.jpg "Western Blot: Apc4 Antibody [NB100-59824] - Detection of Human and Mouse APC4 on HeLa whole cell lysates using NB100-59824. APC4 was also immunoprecipitated by rabbit anti-APC4 antibody NB100-59823.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 758 and 808 of human anaphase-promoting complex subunit 4 using the numbering given in entry NP_037499.2 (GeneID 29945). |
| Predicted Species | Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ANAPC4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Apc4 Antibody (NB100-59824)Find related products by research area.
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| Gene Symbol | ANAPC4 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Imaging overexpressed HA-APC4. Scale bars: 10 μm. (A) HEK293 cells stained with DAPI (blue), GFP (green), HA (magenta), APC4 (red). (B) Wildtype primary mouse hippocampal cultures stained with DAPI (blue), MAP2 (magenta), HA (red), APC4 (green). 1:500 with 36-40hr primary incubation (24 or less does not work for both HEK293 and neurons). The signal that is there is absent in knockout cells. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-59824_rabbit-polyclonal-apc4-antibody-knockout-validated-1610202412455.png "Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Imaging overexpressed HA-APC4. Scale bars: 10 μm. (A) HEK293 cells stained with DAPI (blue), GFP (green), HA (magenta), APC4 (red). (B) Wildtype primary mouse hippocampal cultures stained with DAPI (blue), MAP2 (magenta), HA (red), APC4 (green). 1:500 with 36-40hr primary incubation (24 or less does not work for both HEK293 and neurons). The signal that is there is absent in knockout cells. Image from a verified customer review.")
![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Primary hippocampal neuron cultures were generated from tm1c/tm1c conditional ANAPC4 knockout mice and infected at DIV1 with a lentivirus expressing Cre RFP P2A or a No Infection control. Coverslips were fixed at DIV11 and stained with antibodies against MAP2 (blue), Cre (green), and APC4 (magenta). The RFP expression was also detected (red). The settings on the confocal microscope were determined by using a non-infected control sample that was not stained with the APC4 primary antibody (Secondary Only). Scale bars representing 10 um are displayed on the merged images (Merge). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-59824_rabbit-polyclonal-apc4-antibody-knockout-validated-1610202412939.png "Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal Apc4 Antibody [NB100-59824] - Primary hippocampal neuron cultures were generated from tm1c/tm1c conditional ANAPC4 knockout mice and infected at DIV1 with a lentivirus expressing Cre RFP P2A or a No Infection control. Coverslips were fixed at DIV11 and stained with antibodies against MAP2 (blue), Cre (green), and APC4 (magenta). The RFP expression was also detected (red). The settings on the confocal microscope were determined by using a non-infected control sample that was not stained with the APC4 primary antibody (Secondary Only). Scale bars representing 10 um are displayed on the merged images (Merge). Image from a verified customer review.")
![Western Blot Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Apolipoprotein_C-II_AF4497_Western_Blot_5478.jpg)
![Simple Western Apolipoprotein C-II/ApoC2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Apolipoprotein_CII_AF4497_Simple_Western_17514.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")