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Annexin A2 Antibody (1G7)

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 (1 Review)
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Immunocytochemistry/Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) and annexin A2 (Alexa 647-conjugated secondary antibody, red). An enlarged inset (white dotted line) is included for prompt visualization. Nuclei are visualized with DAPI (blue); scale bar, 10um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-03470-w), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Localization of annexin A2 at the focal adhesions: KS1767 cells co-stained for paxillin (FITC-conjugated secondary antibody, green) ...read more
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Co-localization analysis: KS1767 cells grown overnight onto circular coverslips were incubated for 2h at 37degrees C with biotinylated ...read more
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - MDA-MB-231 subclones whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. ANXA2 Antibody (H00000302-M02) was used for primary antibody: 1:2000, 4C, ...read more
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - ANXA2 monoclonal antibody (M02), clone 1G7 Analysis of ANXA2 expression in HepG2.
Western Blot: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M02), clone 1G7.Lane 1: ANXA2 transfected lysate(40.4 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: Annexin A2 Antibody (1G7) [H00000302-M02] - Analysis of monoclonal antibody to ANXA2 on HeLa cell. Antibody concentration 10 ug/ml.
ANXA2 could regulate the activation of NF-kappaB through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-alpha (100 ng/ml) ...read more
NDRG2 decreases the glucose uptake and GLUT1 protein levels in SK-BR-3-based subcutaneously xenograft tumours. The experiments illustrated are described in the Methods section. (A) Tumour growth was assessed every 3 ...read more
Fibrillar adhesions and FN fibrils are dynamic structures governed by Rab21 and PPFIA1.(a) Living siCTL and siRAB21 ECs were incubated with IST9 mAb for 30 min, fixed, acid washed and stained. RAB21 silencing impairs ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IP
Clone
1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Annexin A2 Antibody (1G7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ANXA2 (AAH66955, 19 a.a. ~ 357 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISVMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Specificity
ANXA2 - annexin A2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ANXA2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00000302-M02 in the following applications:
Publications
Read Publications using
H00000302-M02 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28652618).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Annexin A2 Antibody (1G7)

  • Annexin A2
  • Annexin II
  • annexin-2
  • ANX2
  • ANX2L4
  • ANX2L4LPC2
  • ANXA2
  • CAL1H
  • Calpactin I heavy chain
  • calpactin I heavy polypeptide
  • Calpactin-1 heavy chain
  • chromobindin 8
  • chromobindin-8
  • LIP2
  • LIP2PAP-IV
  • Lipocortin II
  • Lipocortin-2
  • LPC2D
  • LPC2DP36
  • p36
  • PAP-IV
  • Placental anticoagulant protein IV
  • Protein I

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Annexin A2 Antibody (H00000302-M02)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
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Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using H00000302-M02 Applications Species
Tramutola A, Abate G, Lanzillotta C et al. Protein nitration profile of CD3+ lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection. Free Radic Biol Med 2018-10-12 [PMID: 30321702]
Wang Y, Chen K, Cai Y et al. Annexin A2 could enhance multidrug resistance by regulating NF-?B signaling pathway in pediatric neuroblastoma Journal of Experimental & Clinical Cancer Research 2017-12-01 [PMID: 28814318] (ICC/IF) ICC/IF
Na Pombejra S, Salemi M, Phinney BS, Gelli A et al. The Metalloprotease, Mpr1, Engages AnnexinA2 to Promote the Transcytosis of Fungal Cells across the Blood-Brain Barrier. Front Cell Infect Microbiol 2017-06-30 [PMID: 28713781]
Staquicini DI, Rangel R, Guzman-Rojas L et al. Intracellular targeting of annexin A2 inhibits tumor cell adhesion, migration, and in vivo grafting Sci Rep 2017-06-26 [PMID: 28652618] (ICC/IF, Mouse) ICC/IF Mouse
Zakrzewicz D, Didiasova M, Zakrzewicz A et al. The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization. Biochem J. 2014-06-01 [PMID: 24628430]
Deng FY, Lei SF, Zhang Y et al. Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans. Mol Cell Proteomics. 2011-08-04 [PMID: 21817168]
Menegay M, Lee D, Tabbara KF et al. Proteomic Analysis of Climatic Keratopathy Droplets. Invest Ophthalmol Vis Sci;49(7):2829-2837. 2008-01-01 [PMID: 18378572]

Review for Annexin A2 Antibody (H00000302-M02) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00000302-M02:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
 WB Human 09/12/2021
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231
SpeciesHuman
LotH1121-1G7

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Western Blot Annexin A2 H00000302-M02
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 Yongkang Yang
09/12/2021
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ANXA2
Entrez
OMIM
Uniprot